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Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db Antibody

Pricing & Availability
Clone
28-8-6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H) IgG2a, κ
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Product Citations
publications
28-8-6_Alx647_122607
C57BL/6 mouse splenocytes stained with 28-8-6 Alexa Fluor® 647
  • 28-8-6_Alx647_122607
    C57BL/6 mouse splenocytes stained with 28-8-6 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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114612 100 µg 184€
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Description

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
  2. Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
  3. Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
  4. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations
  1. Capietto AH, et al. 2020. J Exp Med. 217:00:00. PubMed
  2. Santharam MA, et al. 2023. Int J Mol Sci. 24:. PubMed
  3. Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed
  4. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  5. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  6. Li J, et al. 2020. Cancer Discov. . PubMed
  7. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  8. Li J, et al. 2018. Immunity. 49:178. PubMed
  9. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  10. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  11. Bui J, et al. 2006. J Immunol. 176:905. PubMed
RRID
AB_492931 (BioLegend Cat. No. 114612)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb/H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kb/H-2Db on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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