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Alexa Fluor® 647 anti-mouse Lypd8 Antibody

Pricing & Availability
Clone
SA025A4 (See other available formats)
Regulatory Status
RUO
Other Names
Ly6/PLAUR domain containing 8
Isotype
Mouse IgG1, κ
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Product Citations
publications
SA025A4_A647_Lypd8_Antibody_112017
C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/mL of Alexa Fluor® 647 Lypd8 (clone SA025A4) overnight at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • SA025A4_A647_Lypd8_Antibody_112017
    C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/mL of Alexa Fluor® 647 Lypd8 (clone SA025A4) overnight at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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152705 25 µg 128€
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152706 100 µg 306€
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Description

Ly6/PLAUR domain containing 8 (Lypd8) is a 100-120 kD GPI-anchored glycoprotein and a member of the Ly6/PLAUR family. Lypd8 is highly expressed on epithelial cells from intestinal lumen, where it prevents bacterial invasion by inhibiting bacterial motility by binding to flagella.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Lypd8-transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration of 2.5 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

​* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This clone does not recognize Lypd8 from BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Okumura R, et al. 2016. Nature 532:117.
RRID
AB_2721428 (BioLegend Cat. No. 152705)
AB_2721428 (BioLegend Cat. No. 152706)

Antigen Details

Structure
GPI-anchored, glycoprotein, 100-120 kD.
Distribution

Epitelials cells in cecum and large intestine.

Function
Inhibits bacterial motility by binding to flagella.
Cell Type
Epithelial cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Okumura R, et al. 2016. Nature 532:117.
2. Loughner CL, et al. 2016. Hum. Genomics 10:10.

Gene ID
70163 View all products for this Gene ID
UniProt
View information about Lypd8 on UniProt.org
Go To Top Version: 0    Revision Date: 11.20.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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