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Alexa Fluor® 647 anti-PTEN Phospho (Ser380) Antibody

Pricing & Availability
Clone
A22005D (See other available formats)
Regulatory Status
RUO
Other Names
Phosphatase and tensin homolog, PTEN1, BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A22005D_A-647_PTEN_ICFC_072224
Serum starved SR cells either untreated (positive control, filled histogram), or treated with 4,5,6,7-Tetrabromobenzotriazole (TBB) (negative control, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-PTEN Phospho (Ser380) (clone A22005D) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400155).
  • A. A22005D_A-647_PTEN_ICFC_072224
    Serum starved SR cells either untreated (positive control, filled histogram), or treated with 4,5,6,7-Tetrabromobenzotriazole (TBB) (negative control, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-PTEN Phospho (Ser380) (clone A22005D) or Alexa Fluor® 647 mouse IgG1, κ isotype control (open histogram, dashed line) (representative histogram for either untreated or treated cells) (Cat. No. 400155).
  • B. A22005D_A-647_PTEN_IHC-P_072224
    IHC staining with Alexa Fluor® 647 anti-PTEN Phospho (Ser380) was performed on formalin-fixed paraffin-embedded human colon adenocarcinoma following antigen retrieval using Tris-EDTA pH 9.0 buffer (Cat. No. 422703). The tissue sections were incubated with Alexa Fluor® 647 secondary antibody only (panel A) or with Alexa Fluor® 647 anti-PTEN Phospho (Ser380) (clone A22005D) (panel B). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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626453 25 µg 144€
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Description

PTEN is a tumor suppressor gene which encodes a phosphatase that preferentially dephosphorylates phosphoinositide substrates and thereby modulates cell cycle progression and cell survival. PTEN is an important negative regulator of the PI3K/AKT/mTOR signaling pathway by dephosphorylating PIP3. PTEN is commonly mutated in human cancer, hence, loss of expression/function of PTEN due to mutations have been observed in prostate cancer, glioblastoma, endometrial, lung and breast cancers. Other non-cancerous clinical disorders associated with PTEN mutation are Cowden syndrome, Bannayan-Riley-Ruvalcaba syndrome, and proteus-like syndrome. PTEN is phosphorylated at several residues and some lead to increase in activity while others lead to loss of function and degradation. Specifically, phosphorylation at serine 380 residue by casein kinase 2 (CK2), leads to degradation of PTEN in the proteasome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human PTEN phosphorylated at Serine 280
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

For use of this antibody (clone A22005D) in intracellular staining in flow cytometry (ICFC), we recommend using True-Phos ™ Perm Buffer (Cat. No. 425401) for fixation/permeabilization.

For use of this antibody (clone A22005D) in immunohistochemistry on FFPE tissues (IHC-P), we recommend antigen retrieval with Tris-EDTA pH 9.0 Buffer (Cat. No. 422703). 

RRID
AB_3662411 (BioLegend Cat. No. 626453)

Antigen Details

Structure
PTEN is a 403 amino acid protein with a predicted molecular weight of 55 kD. It has a tyrosine phosphatase domain and a C2 domain
Distribution

Cytoplasmic (Inner cellular membrane) and nuclear

Function
PTEN functions as a lipid and protein phosphatase. It is negatively regulated by phosphorylation at the C-terminal domain which leads to proteasomal degradation.
Interaction
P53, PTK2, MAGI3, CSNK2A2, NEDD4, NR3C4, MVP
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Dempsey DR, et al. 2021. Nature Structural & Molecular Biology. 28:858-868.
  2. Gupta A, et al. 2016. JBC. 291:18465-73.
  3. Song MS, et al. 2012. Nature Reviews Molecular Cell Biology. 13:283-96.
  4. Vazquez F, et al. 2000. Mol Cell Biol. 20:5010-8. 
Gene ID
5728 View all products for this Gene ID
UniProt
View information about PTEN Phospho Ser380 on UniProt.org
Go To Top Version: 1    Revision Date: 07.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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