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Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AD2_Alexa-Fluour-700_CD73_Antibody_112122
Human peripheral blood lymphocytes were stained with anti-human CD3 PE and anti-human CD73 (Ecto-5’-nucleotidase) (clone AD2) Alexa Fluor® 700 (left) or mouse IgG1, κ Alexa Fluor® 700 isotype control (right).
  • AD2_Alexa-Fluour-700_CD73_Antibody_112122
    Human peripheral blood lymphocytes were stained with anti-human CD3 PE and anti-human CD73 (Ecto-5’-nucleotidase) (clone AD2) Alexa Fluor® 700 (left) or mouse IgG1, κ Alexa Fluor® 700 isotype control (right).
Compare all formats See Alexa Fluor® 700 spectral data
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344041 25 tests 137€
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344042 100 tests 300€
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Wilson A, et al. 2023. F1000Res. 1280:11. PubMed
RRID
AB_2927998 (BioLegend Cat. No. 344041)
AB_2927998 (BioLegend Cat. No. 344042)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD73 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F
Purified anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F,Block
PE anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Biotin anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™) AD2 FC
PerCP/Fire™ 806 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark PLUS B550™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Go To Top Version: 1    Revision Date: 11.23.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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