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Alexa Fluor® 700 anti-human CD81 (TAPA-1) Antibody

Pricing & Availability
Clone
5A6 (See other available formats)
Regulatory Status
RUO
Other Names
S5.7, CVID6, TSPAN28
Isotype
Mouse IgG1, κ
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Product Citations
publications
5A6_A700_CD81_Antibody_041717
Human peripheral blood lymphocytes were stained with CD81 (clone 5A6) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ isotype control Alexa Fluor® 700 (open histogram).
  • 5A6_A700_CD81_Antibody_041717
    Human peripheral blood lymphocytes were stained with CD81 (clone 5A6) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ isotype control Alexa Fluor® 700 (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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349517 25 tests 128€
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349518 100 tests 372€
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Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human OCI-LY8 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP)
  3. Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB)
  4. Mochida K, et al. 2008. J. Virol. 13:6711.
  5. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
Product Citations
  1. Newman LA, et al. 2021. Cells. 10:. PubMed
RRID
AB_2687128 (BioLegend Cat. No. 349517)
AB_2687128 (BioLegend Cat. No. 349518)

Antigen Details

Structure
26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution

T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.

Function
Regulates cell activation and growth and cell aggregation.
Cell Type
B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127.
  3. Wright M, et al. 1994. Immunol. Today 15:588.
Gene ID
975 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org
Go To Top Version: 0    Revision Date: 04.17.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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