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Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
W21115A (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21115A_A647_CD326_Antibody-1_100424
IHC staining of anti-mouse CD326 (Ep-CAM) (clone W21115A) Alexa Fluor® 647 on C57BL/6 mouse frozen uterine tissue. After fixing with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, the tissue was blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was stained with 5 µlg/mL of rat IgG2a, κ Alexa Fluor® 647 isotype control (panel A) or anti-mouse CD326 (Ep-CAM) Alexa Fluor® 647 (panel B) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. Scale bar: 50 µm
  • A. W21115A_A647_CD326_Antibody-1_100424
    IHC staining of anti-mouse CD326 (Ep-CAM) (clone W21115A) Alexa Fluor® 647 on C57BL/6 mouse frozen uterine tissue. After fixing with 4% paraformaldehyde (PFA) for 10 minutes at room temperature, the tissue was blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was stained with 5 µlg/mL of rat IgG2a, κ Alexa Fluor® 647 isotype control (panel A) or anti-mouse CD326 (Ep-CAM) Alexa Fluor® 647 (panel B) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. Scale bar: 50 µm
  • B. W21115A_A647_CD326_Antibody-2_100424
    IHC staining of anti-mouse CD326 (Ep-CAM) (clone W21115A) Alexa Fluor° 647 on formalin-fixed paraffin-embedded C57BL/6 mouse small intestine tissue. After antigen retrieval using 1X Citrate Buffer (Cat. No. 420901), the tissue was stained with 2.5 µlg/mL of rat IgG2a, κ Alexa Fluor® 647 isotype control (panel C) or anti-mouse CD326 (Ep-CAM) Alexa Fluor® 647 (panel D) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. Scale bar: 50 µm
  • C. W21115A_A647_CD326_Antibody-3_100424
    IHC staining of anti-mouse CD326 (Ep-CAM) (clone W21115A) Alexa Fluor® 647 on formalin-fixed paraffin-embedded C57BL/6 mouse uterine tissue. After antigen retrieval using 1X Citrate Buffer 1X (Cat. No. 420901), the tissue was stained with 5 µg/mL of rat IgG2a, κ Alexa Fluor® 647 isotype control (panel E) or anti-mouse CD326 (Ep-CAM) Alexa Fluor® 647 (panel F) (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. Scale bar: 50 µm
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114205 25 µg 156€
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114206 100 µg 356€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein aa24-266
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Function
Mediates calcium-independent homophilic cell-cell adhesion
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.04.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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