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Anti-BACE1 (CO2 terminus) Antibody (Previously Covance catalog# PRB-617C)

Pricing & Availability
Clone
Poly8401 (See other available formats)
Regulatory Status
RUO
Other Names
Beta-Secretase 1, Asp 2, Memapsin-2, App Beta-Secretase, Aspartyl Protease 2, Beta-Site App Cleaving Enzyme 1, Beta-Secretase 1 Precursor Variant 1, Transmembrane Aspartic Proteinase Asp2, Membrane-Associated Aspartic Protease 2, Amyloid Beta A4
Previously
Covance Catalog# PRB-617C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Purified_BACE1_Antibody_IHC_1_030415
BACE subcellular localization in rat primary cortical neurons detected by BACE1 antibody B690 used at a 1:300 dilution. Photo courtesy of H. Xu, The Burnham Institute.
  • Purified_BACE1_Antibody_IHC_1_030415
    BACE subcellular localization in rat primary cortical neurons detected by BACE1 antibody B690 used at a 1:300 dilution. Photo courtesy of H. Xu, The Burnham Institute.
  • Purified_BACE1_Antibody_IHC_2_030415
    On the left, stable N2a cells overexpressing human BACE1 are immunprecipitated with B690 at a 1:250 dilution. The IP is detected on the blot with GP45 anti-BACE1. On the right, anti-BACE1 B690 used at 1:1,000 detects 2 bands on a standard Western blot of N2a cell lysates stably expressing human BACE1. Photo courtesy of H. Xu, The Burnham Institute.
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840101 100 µL 336€
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Description

Beta-secretase 1 (BACE1) is a novel membrane bound aspartyl protease that cleaves the β-amyloid precursor protein (βAPP) to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase, resulting in the generation and secretion of the Aβ. The amyloidogenic peptide Aβ is a major factor in the etiology of Alzheimer’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
B690 was raised against a peptide sequence CLRQQHDDFADDISLLK , the C-terminal 17 amino acids of the human BACE1 and recognizes the mature and immature forms of BACE.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, ICC, IP
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000
• ICC: 1:300
• IP: 1:200

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry, (ICC) and immunoprecipitation (IP).

Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease which cleaves the β-APP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase, resulting in the generation and secretion of Aβ.

Clone also known as B690.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Yan R, Han P, Miao H, Greengard P, Xu H. The transmembrane domain of the Alzheimer's beta-secretase (BACEdetermines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate. J Biol Chem 276(39):36788-36796, 2001. (WB, IP)
  2. Lysenko LV, et al. 2014. PLoS One.9:114521. (WB) PubMed
Product Citations
  1. Mohan ML, et al. 2013. Sci Signal. ra4:6. PubMed
  2. Liu L, et al. 2019. J Cell Biol. 218:644. PubMed
  3. Lysenko L, et al. 2014. PLoS One. 9:e114521. PubMed
RRID
AB_2565445 (BioLegend Cat. No. 840101)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Gene ID
23621 View all products for this Gene ID
UniProt
View information about BACE1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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