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Anti-Neurofilament M (NF-M) Antibody (Previously Covance catalog# PRB-575C)

Pricing & Availability
Clone
Poly28410 (See other available formats)
Regulatory Status
RUO
Other Names
160 kD Neurofilament Protein, Neurofilament Triplet M Protein, Neurofilament-3 (150 Kd Medium), Neurofilament, Medium Polypeptide 150kD
Previously
Covance Catalog# PRB-575C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly28410_Neurofilament-M_Antibody_IF_1_032119.png
IHC staining of anti-Neurofilament M (NF-M) antibody (Poly28410) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:200 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 donkey anti-rabbit IgG (Cat. No. 406414) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1_Poly28410_Neurofilament-M_Antibody_IF_1_032119.png
    IHC staining of anti-Neurofilament M (NF-M) antibody (Poly28410) on formalin-fixed paraffin-embedded mouse cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:200 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 donkey anti-rabbit IgG (Cat. No. 406414) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2_Poly28410_Neurofilament-M_Antibody_IF_2_032119.png
    IHC staining of anti-Neurofilament M (NF-M) antibody (Poly28410) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:200 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 donkey anti-rabbit IgG (Cat. No. 406414) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 3_Poly28410_Neurofilament-M_Antibody_IF_3_032119.png
    IHC staining of anti-Neurofilament M (NF-M) antibody (Poly28410) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:200 dilution of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 donkey anti-rabbit IgG (Cat. No. 406414) for one hour at room temperature. The slide was mounted with Fluoromount-G with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 4_Poly28410_Neurofilament-M_Antibody_5_040319.png
    IHC staining of anti-Neurofilament M (NF-M) antibody (Poly28410) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with a 1:500 dilution of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5_Poly28410_Neurofilament-M_Antibody_4_040319.png
    Western blot of anti-Neurofilament M (NF-M) antibody (Poly28410). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain lysate; Lane 3: 30 µg of mouse brain lysate; Lane 4: 30 µg of rat brain lysate. The blot was incubated with a 1:10,000 dilution of the primary antibody overnight at 4°C, followed by incubation with HRP anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
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841001 100 µL 251€
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Description

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies in the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Rat, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The immunogen was the extreme carboxyterminal region of rat NF-M, which was expressed in E. coli and purified by ion exchange chromatograpy.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution range of 1:200 - 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:5000 - 1:10000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The production and characterization of this antiserum similar to monoclonal NF-M clone 3H11.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Harris J, Ayyub C, Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J. Neurosci. Res. 30:47-62, 1991.
Product Citations
  1. Vieira JR, et al. 2022. Neuron. 110:4074. PubMed
  2. Homma S, et al. 2023. Front Neurosci. 16:1009542. PubMed
  3. Min S, et al. 2020. Cell Reports. 29(8):2192-2201.e3.. PubMed
  4. Paredes I, et al. 2021. Nat Neurosci. 24:478. PubMed
  5. Xu R, et al. 2018. Nat Med. 24:823. PubMed
  6. Kirst C, et al. 2020. Cell. 180(4):780-795. PubMed
RRID
AB_2565457 (BioLegend Cat. No. 841001)

Antigen Details

Structure
Expected MW: 145-160 kD
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
4741 View all products for this Gene ID
UniProt
View information about Neurofilament M on UniProt.org
Go To Top Version: 5    Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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