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APC anti-human CD226 (DNAM-1) Antibody

Pricing & Availability
Clone
11A8 (See other available formats)
Regulatory Status
RUO
Other Names
DNAM-1, PTA1, TLiSA1
Isotype
Mouse IgG1, κ
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Product Citations
publications
11A8_APC_CD226_DNAM-1_Antibody_1_081117
Human peripheral blood lymphocytes were stained with CD3 Brilliant Violet 421™ and CD226 (clone 11A8) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 11A8_APC_CD226_DNAM-1_Antibody_1_081117
    Human peripheral blood lymphocytes were stained with CD3 Brilliant Violet 421™ and CD226 (clone 11A8) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 11A8_APC_CD226_DNAM-1_Antibody_2_081117
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338311 25 tests 91€
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338312 100 tests 194€
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Description

DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: block NK cell-mediated lysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Fuchs A, et al. 2004. J. Immunol. 172:3994.
Product Citations
  1. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  2. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  3. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  4. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
  5. Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
  6. Sutavani R, et al. 2013. J Immunol. 191:5895. PubMed
  7. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
  8. Choi JW, et al. 2019. BMC Cancer. 19:817. PubMed
  9. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  10. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  11. Alonso R, et al. 2022. Blood Adv. :. PubMed
  12. Elias S, et al. 2020. Haematologica. . PubMed
RRID
AB_2561951 (BioLegend Cat. No. 338311)
AB_2561951 (BioLegend Cat. No. 338312)

Antigen Details

Structure
Ig domain containing adhesion molecule
Distribution

Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells

Function
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
Interaction
Interacts with LFA-1.
Ligand/Receptor
PVR (CD155) and Nectin-2 (CD112)
Cell Type
B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Shibuya A et al. 1996. Immunity 4:573.
2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533.
3. Pende D et al. 2005. Mol. Immunol. 42:463.

Gene ID
10666 View all products for this Gene ID
UniProt
View information about CD226 on UniProt.org
Go To Top Version: 2    Revision Date: 08.11.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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