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APC anti-human CD59 Antibody

Pricing & Availability
Clone
p282 (H19) (See other available formats)
Regulatory Status
RUO
Workshop
V S006
Other Names
Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P282_APC_CD59_Antibody_062719.png
Human peripheral blood lymphocytes were stained with anti-human CD59 (clone P282) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • P282_APC_CD59_Antibody_062719.png
    Human peripheral blood lymphocytes were stained with anti-human CD59 (clone P282) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
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304711 25 tests 91€
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304712 100 tests 214€
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Description

CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Baboon, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Park J, et al. 2021. Exp Ther Med. 22:808. PubMed
  2. Chen P, et al. 2021. Front Immunol. 12:689019. PubMed
RRID
AB_2819929 (BioLegend Cat. No. 304711)
AB_2819929 (BioLegend Cat. No. 304712)

Antigen Details

Structure
Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
Distribution

All cell types

Function
Blocks complement-mediated lysis by inhibiting MAC formation
Ligand/Receptor
Complement components C8, C9
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Davies A, et al. 1993. Immunol. Res. 12:258.
2. Lachmann P. 1991. Immunol. Today 12:312.
3. Liszewski M, et al. 1996. Adv. Immunol. 61:201.

Gene ID
966 View all products for this Gene ID
UniProt
View information about CD59 on UniProt.org
Go To Top Version: 1    Revision Date: 06.27.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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