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APC anti-IRF6 Antibody

Pricing & Availability
Clone
14B2C16 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 6, IRF-6
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
14B2C16_APC_IRF6_Antibody_022822
HaCat cells were treated with Fixation Buffer (Cat. No. 420801) and permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-IRF6 (clone 14B2C16) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • 14B2C16_APC_IRF6_Antibody_022822
    HaCat cells were treated with Fixation Buffer (Cat. No. 420801) and permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-IRF6 (clone 14B2C16) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
See APC spectral data
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674507 25 tests 133€
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674508 100 tests 304€
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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2910495 (BioLegend Cat. No. 674507)
AB_2910495 (BioLegend Cat. No. 674508)

Antigen Details

Structure
467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution

Nucleus and cytoplasm.

Function
IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction
Interacts with SERPINB5.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.

Gene ID
3664 View all products for this Gene ID
UniProt
View information about IRF6 on UniProt.org

Other Formats

View All IRF6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-IRF6 14B2C16 WB,ICFC,ICC
APC anti-IRF6 14B2C16 ICFC
Go To Top Version: 1    Revision Date: 02.28.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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