APC anti-mouse CD20 Antibody, CD20, SA271G2

Pricing & Availability

Clone: SA271G2 (See other available formats)
Regulatory Status: RUO
Other Names: Ms4a1, Ly-44
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with CD45R/B220 APC/Cyanine7 and CD20 (clone SA271G2) APC (left) or rat IgG2b, κ APC isotype control (right).

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152107

25 µg

120€

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152108

100 µg

280€

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Description

CD20 is a 33-37 kD protein, a member of the MS4A family, with four transmembrane spanning regions that present as a homo-oligomeric complexes in the cell surface when associating with MHC class I and II, CD53, CD81, and CD82. CD20 is expressed on B cells and a subset of T cells, but not on plasma cells. CD20 regulates B-cell activation and proliferation. Its ligation promotes transmembrane Ca²⁺ trafficking. CD20 is an important therapeutic target in the treatment of B cell lymphomas and leukemias.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD20 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
RRID: AB_2819903 (BioLegend Cat. No. 152107)
AB_2819903 (BioLegend Cat. No. 152108)

Antigen Details

Structure: Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree.
Distribution: B cells and a subset of T cells
Function: Regulates B-cell activation and proliferation
Interaction: Associates with MHC class I and II, CD53, CD81, and CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Morsy DE, et al. 2013. J. Immunol. 191:3112.
2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236.
3.Beers SA, et al. 2010. Blood 115:5191.
4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214.
Gene ID: 12482 View all products for this Gene ID
UniProt: View information about CD20 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

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Other Formats

View All CD20 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD20	SA271G2	FC,Depletion
Ultra-LEAF™ Purified anti-mouse CD20	SA271G2	FC,Depletion
PE anti-mouse CD20	SA271G2	FC
APC anti-mouse CD20	SA271G2	FC
PE/Dazzle™ 594 anti-mouse CD20 Antibody	SA271G2	FC
FITC anti-mouse CD20	SA271G2	FC
PE/Cyanine5 anti-mouse CD20	SA271G2	FC
APC/Fire™ 750 anti-mouse CD20	SA271G2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 12.10.2019

For Research Use Only. Not for diagnostic or therapeutic use.

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