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APC anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_APC_052610
Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 APC
  • FA-11_APC_052610
    Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 APC
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137007 25 µg 110€
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137008 100 µg 261€
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Murotomi K, et al. 2022. Exp Biol Med (Maywood). 247:848. PubMed
  2. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  3. Wang X, et al. 2017. Br J Pharmacol. 10.1111/bph.13786. PubMed
  4. Tang Q, et al. 2023. Heliyon. 9:e14006. PubMed
  5. Aguilar-Pineda JA, et al. 2021. iScience. 24:102993. PubMed
  6. Yamane M, et al. 2020. FASEB J. 34:10778. PubMed
  7. Sato S, et al. 2021. Int J Mol Sci. 22:. PubMed
  8. Zheng L, et al. 2022. J Neuroinflammation. 19:128. PubMed
  9. Izquierdo-Useros N, et al. 2014. J Immunol . 192:4852-4858. PubMed
  10. Krishack P, et al. 2015. J Am Heart Assoc. 4: 001858. PubMed
  11. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  12. Li H, et al. 2022. Gut Microbes. 14:2127456. PubMed
  13. Guo J, et al. 2020. J Immunol. 205:2916. PubMed
  14. Lu J, et al. 2021. Cell Death Discov. 7:165. PubMed
  15. Frenis K, et al. 2021. Basic Res Cardiol. 31:116. PubMed
  16. Wang Y et al. 2017. Cell. 171(2):331-345 . PubMed
  17. Senthil K, et al. 2021. Invest Ophthalmol Vis Sci. 62:38. PubMed
  18. Shan B, et al. 2020. Nat Metab. 1332:2. PubMed
  19. Zuo Y, et al. 2020. Small GTPases. 0.661805556. PubMed
  20. Morishita Y, et al. 2016. Sci Rep. 6:21705. PubMed
  21. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  22. Li S, et al. 2019. Front Neurol. 1.036805556. PubMed
  23. Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed
  24. Kanemaru H, et al. 2017. Proc Natl Acad Sci U S A. 114:E7331. PubMed
  25. Mantsounga CS, et al. 2022. Cell Rep. 38:110309. PubMed
RRID
AB_10575299 (BioLegend Cat. No. 137007)
AB_10575299 (BioLegend Cat. No. 137008)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.26.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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