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APC anti-mouse Ig light chain λ Antibody

Pricing & Availability
Clone
RML-42 (See other available formats)
Regulatory Status
RUO
Other Names
Imunoglobulin light chain lambda
Isotype
Rat IgG2a, κ
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Product Citations
publications
RML-42_APC_111407
C57BL/6 mouse splenocytes stained with RML-42 APC
  • RML-42_APC_111407
    C57BL/6 mouse splenocytes stained with RML-42 APC
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407306 100 µg 252€
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Description

The RML-42 monoclonal antibody reacts with immunoglobulin light chain lambda in all tested mouse haplotype (Igh-a and b). It does not react with kappa chain. The RML-42 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ota M, et al. 2010. J. Immunol. 185:4128. PubMed
  2. Xu Z, et al. 2012. J. Immunol. 189:4470. PubMed
Product Citations
  1. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  2. Xu Z, et al. 2012. J Immunol. 189:4470. PubMed
  3. Peter AS, et al. 2021. Eur J Immunol. . PubMed
  4. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  5. Blanc P, et al. 2016. Nat Commun. 7:13600. PubMed
  6. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  7. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  8. Li X, et al. 2018. Immunity. 48:530. PubMed
  9. Ota M, et al. 2010. J Immunol. 185:4128. PubMed
  10. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
RRID
AB_961363 (BioLegend Cat. No. 407306)

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Go To Top Version: 4    Revision Date: 06.11.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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