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APC/Cyanine7 anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5_APCCy7_020608
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC/Cyanine7 (filled histogram) or rat IgG2a, κ APC/Cyanine7 isotype control (open histogram).
  • RM4-5_APCCy7_020608
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC/Cyanine7 (filled histogram) or rat IgG2a, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
100525 25 µg 71€
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100526 100 µg 159€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  2. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  3. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  4. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  5. Kim JS, et al. 2023. J Biol Chem. 299:102886. PubMed
  6. Xiao R, et al. 2022. J Immunol. 208:2109. PubMed
  7. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
  8. Jarick KJ, et al. 2018. Front Immunol. 9:1468. PubMed
  9. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  10. Ho HM, et al. 2022. Pharmaceutics. 14:. PubMed
  11. Ke F, et al. 2023. Elife. 12:. PubMed
  12. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  13. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
  14. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  15. Ma F, et al. 2020. Cell Death Dis. 1.172222222. PubMed
  16. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  17. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  18. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  19. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  20. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  21. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  22. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  23. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  24. Guo HF, et al. 2021. Commun Biol. 4:482. PubMed
  25. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  26. Wei M, et al. 2014. Protein Eng Des Sel. 27:289. PubMed
  27. Westfall S, et al. 2020. Brain Behav Immun. . PubMed
  28. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  29. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  30. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  31. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  32. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  33. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  34. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  35. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  36. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  37. Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed
  38. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  39. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  40. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  41. De Ponte Conti B, et al. 2021. Elife. 10:. PubMed
  42. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  43. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  44. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  45. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  46. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  47. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  48. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  49. Saigusa R, et al. 2018. Arthritis Res Ther. 20:23. PubMed
  50. Boehm DT, et al. 2019. NPJ Vaccines. 4:40. PubMed
  51. Clement CC, et al. 2021. Immunity. 54:721. PubMed
  52. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  53. Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed
  54. Koide S, et al. 2022. iScience. 25:103603. PubMed
  55. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  56. Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed
  57. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  58. Weng Q, et al. 2018. Cell Regen (Lond). 7:7. PubMed
  59. Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed
  60. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  61. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  62. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  63. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  64. Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed
  65. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  66. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  67. Howie D, et al. 2019. Front Immunol. 10:1860. PubMed
  68. Cobbold SP, et al. 2018. Front Immunol. 9:1381. PubMed
RRID
AB_312726 (BioLegend Cat. No. 100525)
AB_312726 (BioLegend Cat. No. 100526)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 3    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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