APC/Fire™ 810 anti-human CD289 (TLR9) Antibody, CD289, S16013D

Pricing & Availability

Clone: S16013D (See other available formats)
Regulatory Status: RUO
Other Names: CD289, TLR9, Toll-like receptor 9
Isotype: Rat IgG2a, κ
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Product Citations: publications

S16013D_APC-Fire-810_CD289_Antibody_060322 — Human peripheral blood lymphocyte cells were stained with anti-human CD304 Brilliant Violet 421™ and anti-human CD19 PE, fixed, permeabilized, and then intracellularly stained with anti-human CD289 (TLR9) (clone S16013D) APC/Fire™ 810 (left) or rat IgG2a, κ APC/Fire™ 810 isotype control (right).

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Size

Price

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394811

25 tests

150€

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394812

100 tests

331€

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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CD289 (TLR9)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.

Excitation Laser

Red Laser (633 nm)

Application Notes

S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).

RRID

AB_2927844 (BioLegend Cat. No. 394811)
AB_2927844 (BioLegend Cat. No. 394812)

Antigen Details

Distribution: TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).
Ligand/Receptor: TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type: B cells, Dendritic cells
Biology Area: Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References: Hornung V, et al. 2002. J. Immunol. 168:4531.

Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202.

Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID: 54106 View all products for this Gene ID
UniProt: View information about CD289 on UniProt.org

Documentation

Reviews

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

Other Formats

View All CD289 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD289 (TLR9)	S16013D	ICFC,FC
PE anti-human CD289 (TLR9)	S16013D	ICFC,FC
Brilliant Violet 421™ anti-human CD289 (TLR9)	S16013D	ICFC,FC
APC anti-human CD289 (TLR9)	S16013D	ICFC,FC
FITC anti-human CD289 (TLR9)	S16013D	ICFC
APC/Fire™ 810 anti-human CD289 (TLR9)	S16013D	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 06.03.2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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