APC Goat anti-mouse IgG (minimal x-reactivity) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Polyclonal

Host Species

Goat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)

1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
3. Winters T, et al. 2014. EMBO J. 33:1256-70.

Product Citations

1. Gusscott S, et al. 2016. PLoS One. 11: 0161158. PubMed
2. Ecker JW, et al. 2020. Vaccines (Basel). 8:00. PubMed
3. Nielsen OL, et al. 2022. APMIS. 130:359. PubMed
4. Zhang JZ, et al. 2022. Nat Biotechnol. 40:1336. PubMed
5. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
6. Mamula D, et al. 2022. NPJ Parkinsons Dis. 8:171. PubMed
7. Bordoloi D, et al. 2022. JCI Insight. 7: . PubMed
8. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
9. Daradoumis J, et al. 2023. Viruses. 15:. PubMed
10. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
11. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
12. Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed
13. Shmuel-Galia L, et al. 2017. J Biol Chem. 292:13415. PubMed
14. Zhang H, et al. 2017. Virol Sin. 32:503. PubMed
15. Robinson S, et al. 2015. Virus Res. 203:56. PubMed
16. Miao X, et al. 2020. MAbs. 12:1804241. PubMed
17. Baniel CC, et al. 2020. Front Immunol. 1.576388889. PubMed
18. Siddiqui SS, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
19. Tonouchi K, et al. 2020. Int Immunol. 613:32. PubMed
20. Neukirch L, et al. 2019. Oncotarget. 10:1458. PubMed
21. Mellergaard M, et al. 2014. J Biol Chem. 289:20078. PubMed
22. Mellergaard M, et al. 2020. J Biol Chem. 295:11803. PubMed
23. Lee A, et al. 2014. Vet J. 200:103. PubMed
24. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
25. Shlapobersky M, et al. 2012. J Gen Virol. 93:1305. PubMed
26. Schober T, et al. 2017. Nat Commun. 8:14209. PubMed
27. Møller SH, et al. 2020. Front Immunol. 1.825. PubMed
28. Ge J, et al. 2021. Nat Commun. 0.673611111. PubMed
29. Høgh RI, et al. 2020. J Immunol. 1746:204. PubMed
30. González-Tajuelo R, et al. 2020. Front Immunol. 11:588212. PubMed
31. Moogk D, et al. 2016. J Immunol. 197: 644 - 654. PubMed
32. Kinchen J et al. 2018. Cell. 175(2):372-386 . PubMed
33. Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed
34. Denies S, et al. 2014. J Control Release. 187:175. PubMed
35. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
36. Mao D, et al. 2022. iScience. 25:103843. PubMed
37. Tsao LC, et al. 2022. JCI Insight. . PubMed
38. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
39. Capelle CM, et al. 2021. iScience. 24:102289. PubMed
40. Satchwell T, et al. 2016. Haematologica. 101: 1018 - 1027. PubMed
41. Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
42. Kask A, et al. 2010. Vaccine. 28:7483. PubMed
43. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
44. Wang Q, et al. 2020. Cell. 181(4):894-904.e9.. PubMed
45. Ardolino M, et al. 2011. Blood. 117:4778. PubMed
46. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
47. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
48. Liu K, et al. 2020. EMBO Rep. 21:e51444. PubMed
49. Miki K, et al. 2021. Nat Commun. 12:3596. PubMed
50. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
51. Kawamura A, et al. 2016. Sci Rep. 6:19464. PubMed
52. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
53. Uno N, et al. 2015. Mol Ther Nucleic Acids. 4, e272. PubMed
54. Wendt E, et al. 2016. J Immunol. 196: 3910 - 3919. PubMed
55. Wang X, et al. 2016. J Immunol. 196: 4090 - 4099. PubMed
56. Uhlenbrock F, et al. 2015. Mol Immunol. 66: 418-427. PubMed
57. Luteijn RD, et al. 2019. Nature. 573:434. PubMed
58. Wang H, et al. 2019. Proc Natl Acad Sci U S A. 116:988. PubMed
59. Pezhouman A, et al. 2022. Cardiovasc Res. 118:828. PubMed
60. Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed
61. Sun H, et al. 2016. Nat Commun. 7:10339. PubMed
62. Ding S, et al. 2016. PLoS Pathog. 12:e1005929. PubMed
63. Wang X, et al. 2020. Immunity. 328:52. PubMed
64. Chen RE, et al. 2021. Nat Med. 717:27. PubMed
65. Giri T, et al. 2022. Heliyon. 8:e09407. PubMed
66. Jang A, et al. 2021. Front Immunol. 12:701066. PubMed
67. Tam K, et al. 2016. MBio. 7: e02024-16. PubMed

RRID

AB_315011 (BioLegend Cat. No. 405308)

Antigen Details

Distribution

B cells

Gene ID

16059 View all products for this Gene ID

UniProt

View information about IgG on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis