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Biotin anti-TDP43 Antibody

Pricing & Availability
Clone
TDP2H4 (See other available formats)
Regulatory Status
RUO
Other Names
TAR DNA-binding protein-43, TDP 43, TARDBP, ALS10
Isotype
Rat IgG2a
Ave. Rating
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Product Citations
publications
TDP2H4_Biotin_TDP43_Antibody_1_081318
Western blot of Biotin anti-TDP43 antibody (clone TDP2H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain nuclear lysate; Lane 3: 20 µg of mouse brain nuclear lysate; Lane 4: 20 µg of rat brain nuclear lysate. The blot was incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • TDP2H4_Biotin_TDP43_Antibody_1_081318
    Western blot of Biotin anti-TDP43 antibody (clone TDP2H4). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain nuclear lysate; Lane 3: 20 µg of mouse brain nuclear lysate; Lane 4: 20 µg of rat brain nuclear lysate. The blot was incubated with 1 µg/ml of the primary antibody for overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • TDP2H4_Biotin_TDP43_Antibody_2_081318
    IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • TDP2H4_Biotin_TDP43_Antibody_3_081318
    IHC staining of Biotin anti-TDP43 antibody (clone TDP2H4) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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808303 25 µg 137€
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808304 100 µg 315€
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Description

TDP43, also known as TARDBP, was originally identified as a protein that binds to the transactivation response (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome. TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins, which bind single stranded RNA. Recent research has shown that hyperphosphorylated, ubiquitinated, and N-terminally truncated TDP43 is the pathological hallmark lesion in most familial and sporadic forms of FTLD-U and ALS (1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 2.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

Expected Staining Pattern: Nuclear

Application References

(PubMed link indicates BioLegend citation)
  1. Neumann M. 2009. Int. J. Mol. Sci. 10.3390/ijms10010232.
RRID
AB_2750118 (BioLegend Cat. No. 808303)
AB_2750118 (BioLegend Cat. No. 808304)

Antigen Details

Structure
TDP43 is a 414 amino acid protein with an apparent molecular mass of 45 kD.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
TDP43
Gene ID
23435 View all products for this Gene ID 230908 View all products for this Gene ID 100174676 View all products for this Gene ID
UniProt
View information about TDP43 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All TDP43 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-TDP43 TDP2H4 IHC-P,WB,Direct ELISA
Biotin anti-TDP43 TDP2H4 WB,IHC-P
Go To Top Version: 1    Revision Date: 08.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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