- Clone
- NAT105 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD1, PDCD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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367421 | 25 tests | 164€ | ||||
367422 | 100 tests | 322€ |
Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- YT cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.
We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501). -
Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2007. Haematologica 92:1059.
- Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
- Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
- Product Citations
-
- RRID
-
AB_2721517 (BioLegend Cat. No. 367421)
AB_2721517 (BioLegend Cat. No. 367422)
Antigen Details
- Structure
- 55 kD type I transmembrane protein.
- Distribution
-
Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
- Function
- Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
- Interaction
- SHP-1 and SHP-2.
- Ligand/Receptor
- PD-L1 (CD274) and PD-L2 (CD273).
- Cell Type
- B cells, T cells, Thymocytes, Tregs
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.
- Gene ID
- 5133 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD279 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with purifie... Tissue imaged here is the lymphoid nodule/germinal center of... Human paraffin-embedded tonsil tissue slices were prepared w... Western blot analysis of Jurkat (lane 1), PBMC untreated (la... PHA-stimulated (three days) human PBMC were stained with pur... -
PE anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (three days) human PBMC were stained with CD2... Multiplexed IHC staining of PE anti-CD279 (clone NAT105) on ... -
APC anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (three days) human PBMC were stained with CD2... -
Alexa Fluor® 488 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 APC... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
PerCP/Cyanine5.5 anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood mononuclear ce... Human peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine7 anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
FITC anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
APC/Cyanine7 anti-human CD279 (PD-1)
PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Alexa Fluor® 647 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 PE ... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Biotin anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 APC... -
Brilliant Violet 510™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 PE ... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 421™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 PE ... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 711™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 785™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 605™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
Brilliant Violet 650™ anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 FIT... PHA-stimulated (3-day) human peripheral blood lymphocytes we... -
PE/Dazzle™ 594 anti-human CD279 (PD-1)
Human peripheral blood lymphocytes were stained with CD3 APC... -
TotalSeq™-Bn1303 anti-human CD279 (PD-1)
-
PE anti-human CD279
Typical results from PHA-stimulated human peripheral blood l... -
Spark Red™ 718 anti-human CD279 (PD-1) (Flexi-Fluor™)
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