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Brilliant Violet 421™ anti-mouse CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
TY25 (See other available formats)
Regulatory Status
RUO
Other Names
PD-L2, PDL2, B7DC, CD273
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TY25_BV421_CD273_Antibody_031418
Mouse B7-DC transfected BHK cells were stained with CD273 (clone TY25) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotpye control (open histogram).
  • TY25_BV421_CD273_Antibody_031418
    Mouse B7-DC transfected BHK cells were stained with CD273 (clone TY25) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotpye control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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107219 50 µg 194€
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Description

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-DC transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107221-107226).

Application References

(PubMed link indicates BioLegend citation)
  1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
  2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
  3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
  4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
  5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
  6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  8. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  2. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  3. Mao C, et al. 2020. Antib Ther. 0.234027778. PubMed
  4. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2728127 (BioLegend Cat. No. 107219)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 42 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1, uncharacterized receptor
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
58205 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 03.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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