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Brilliant Violet 421™ anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
RMT3-23 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMT3-23_BV421_CD366_Antibody_1_041824
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) PerCP/Fire™ 780 and anti-mouse CD366 (Tim-3) (clone RMT3-23) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
  • RMT3-23_BV421_CD366_Antibody_1_041824
    C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε (clone 145-2C11) PerCP/Fire™ 780 and anti-mouse CD366 (Tim-3) (clone RMT3-23) Brilliant Violet 421™ (left) or rat IgG2a, κ Brilliant Violet 421™ isotype control (right).
Compare all formats See Brilliant Violet 421™ spectral data
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119723 50 µg 234€
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
Product Citations
  1. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  2. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  3. Wanhainen KM, et al. 2022. Cancer Immunol Res. 10:871. PubMed
  4. Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed
  5. Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed
  6. Cribioli E, et al. 2022. Front Immunol. 13:976628. PubMed
  7. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  8. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  9. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  10. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  11. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  12. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  13. Wang X, et al. 2021. Theranostics. 11:209. PubMed
RRID
AB_2616908 (BioLegend Cat. No. 119723)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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