Brilliant Violet 421™ anti-rat CD25 Antibody, CD25, OX-39

Pricing & Availability

Clone: OX-39 (See other available formats)
Regulatory Status: RUO
Other Names: IL-2 receptor chain α, IL-2Rα, low affinity IL-2 receptor chain, Tac, p55, Ly-43
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-39_BV-421_CD25_FC_070224 — Con A stimulated (3-days) rat splenocytes were stained with anti-rat CD25 (clone OX-39) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

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202115

50 µg

200€

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Description

CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat T cell blasts from mixed lymphocyte reactions
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections¹, immunoprecipitation¹, weakly blocks IL-2 binding^{1, 2, 3}, and blocks IL-2 induced epithelial cell migration³.
RRID: AB_3662392 (BioLegend Cat. No. 202115)

Antigen Details

Structure: Type I membrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, thymic and splenic dendritic cells, intestinal epithelial cells
Function: Low affinity receptor for IL-2; associates with IL-2 receptor β (CD122) and γ (CD132) chains to form high affinity receptor
Ligand/Receptor: IL-2
Cell Type: B cells, Dendritic cells, Epithelial cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Digness AU, et al. 1996. Exp.Cell Res. 225:422.
2. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281.
Gene ID: 16184 View all products for this Gene ID
UniProt: View information about CD25 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?: It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD25 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-rat CD25	OX-39	FC
PE anti-rat CD25	OX-39	FC
Alexa Fluor® 488 anti-rat CD25	OX-39	FC
Biotin anti-rat CD25	OX-39	FC
APC anti-rat CD25	OX-39	FC
Brilliant Violet 421™ anti-rat CD25	OX-39	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.02.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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