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Brilliant Violet 605™ anti-human CD158b/j (KIR2DL2/L3/S2) Antibody

Pricing & Availability
Clone
DX27 (See other available formats)
Regulatory Status
RUO
Other Names
CD158b1 (KIR2DL2, p58.2), CD158b2 (KIR2DL3, p58.3), CD158j (KIR2DS2, p50.2), KIR-NKAT2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
DX27_BV605_CD158-bj_Antibody_1_042424
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) PE and anti-human CD158b/j (KIR2DL2/L3/S2) (clone DX27) Brilliant Violet 605™ (left) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (right).
  • DX27_BV605_CD158-bj_Antibody_1_042424
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) PE and anti-human CD158b/j (KIR2DL2/L3/S2) (clone DX27) Brilliant Violet 605™ (left) or mouse IgG2a, κ Brilliant Violet 605™ isotype control (right).
Compare all formats See Brilliant Violet 605™ spectral data
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312623 25 tests 177€
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312624 100 tests 348€
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Description

CD158b is expressed on natural killer cells and a subset of T cells. It is a member of the immunoglobulin superfamily containing two immunoglobulin C2-type domains. Both variants and alternative isoforms of CD158b have been reported. The interaction of CD158b with specific HLA-C antigens on a target cell (HLA-Cw1, HLA-Cw3, HLA-Cw7 alleles, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK cell and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Qulaity tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The DX27 monoclonal antibody reacts with a common epitope of KIR2DL2 (CD158b1, p58.2), KIR2DL3 (CD158b2, p58.3), and KIR2DS2 (CD158j, p50.2). Additional reported applications (for the relevant formats) include: restoring the NK cell cytotoxicity1,5.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  2. Lucas M, et al. 2003. J. Virol. 77:2251.
  3. Goodier M, et al. 2000. J. Immunol. 165:139.
  4. Yawata M, et al. 2002. Immunogenetics 54:543.
  5. Valiante NM, et al. 1997. Immunity 7:739.
RRID
AB_3106179 (BioLegend Cat. No. 312623)
AB_3106179 (BioLegend Cat. No. 312624)

Antigen Details

Structure
Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, type I membrane protein
Distribution

NK cells and a subset of T cells

Function
Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-C antigens on target cell
Ligand/Receptor
HLA-C antigens such as HLA-Cw1, HLA-Cw3, HLA-Cw7
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. Uhrburg M, et al. 1997. Immunity 7:753.
3. Wagtmann N, et al. 1995. Immunity 3:801.
4. Dohring C, et al. 1996. Immunogenetics 44:227.
5. Maenaka K, et al. 1999. Structure 7:391.

Gene ID
3803 View all products for this Gene ID
UniProt
View information about CD158b/j on UniProt.org
Go To Top Version: 1    Revision Date: 04.24.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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