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Brilliant Violet 711™ anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
B8.2C12 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
B8dot2C12_BV711_CD366_Antibody_123020
Mouse CD366 (Tim-3) transfected cells were stained with anti-mouse CD366 (Tim-3) (clone B8.2C12) Brilliant Violet™ 711 (filled histogram) or rat IgG1, κ Brilliant Violet™ 711 (open histogram).
  • B8dot2C12_BV711_CD366_Antibody_123020
    Mouse CD366 (Tim-3) transfected cells were stained with anti-mouse CD366 (Tim-3) (clone B8.2C12) Brilliant Violet™ 711 (filled histogram) or rat IgG1, κ Brilliant Violet™ 711 (open histogram).
Compare all formats See Brilliant Violet 711™ spectral data
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134021 50 µg 243€
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mTim-3 protein/Freund adjuvant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone B8.2C12 only binds to the BALB/c allele of Tim-3.

Application References

(PubMed link indicates BioLegend citation)
  1. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Sanchez-Moral L, et al. 2023. EBioMedicine. 91:104555. PubMed
  2. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID
AB_2890691 (BioLegend Cat. No. 134021)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution

Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.

Function
Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand/Receptor
Galectin -9
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454
3. Mooney L, et al. 2002. Nature. 415:536
4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.30.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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