Brilliant Violet 750™ anti-mouse/human CD44 Antibody, CD44, IM7

Pricing & Availability

Clone: IM7 (See other available formats)
Regulatory Status: RUO
Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype: Rat IgG2b, κ
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IM7_BV750_CD44_Antibody_052623 — Mouse (C57BL/6) splenocytes stained with anti-mouse/human CD44 (clone IM7) Brilliant Violet 750™ (filled histogram) or unstained control only (open histogram).

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103079

50 µg

296€

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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186²⁰. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity¹, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
RRID: AB_2941373 (BioLegend Cat. No. 103079)

Antigen Details

Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type: B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.
Gene ID: 12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt: View information about CD44 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
APC anti-mouse/human CD44	IM7	FC
Biotin anti-mouse/human CD44	IM7	FC,ICC,IHC-P
FITC anti-mouse/human CD44	IM7	FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44	IM7	FC
Purified anti-mouse/human CD44	IM7	FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44	IM7	FC
PE anti-mouse/human CD44	IM7	FC
Alexa Fluor® 488 anti-mouse/human CD44	IM7	FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44	IM7	FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44	IM7	FC
Alexa Fluor® 700 anti-mouse/human CD44	IM7	FC,SB
PE/Cyanine7 anti-mouse/human CD44	IM7	FC
APC/Cyanine7 anti-mouse/human CD44	IM7	FC
PerCP/Cyanine5.5 anti-mouse/human CD44	IM7	FC
PerCP anti-mouse/human CD44	IM7	FC
Brilliant Violet 421™ anti-mouse/human CD44	IM7	FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 785™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 510™ anti-mouse/human CD44	IM7	FC
Ultra-LEAF™ Purified anti-mouse/human CD44	IM7	FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44	IM7	FC
Purified anti-mouse/human CD44 (Maxpar® Ready)	IM7	FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44	IM7	ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44	IM7	FC
Brilliant Violet 711™ anti-mouse/human CD44	IM7	FC
APC/Fire™ 750 anti-mouse/human CD44	IM7	FC
TotalSeq™-A0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-C0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-B0073 anti-mouse/human CD44	IM7	PG
Spark YG™ 570 anti-mouse/human CD44	IM7	FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44	IM7	FC
TotalSeq™-D0073 anti-mouse/human CD44	IM7	PG
Brilliant Violet 750™ anti-mouse/human CD44	IM7	FC
PerCP/Fire™ 806 anti-mouse/human CD44	IM7	FC
Spark Red™ 718 anti-mouse/human CD44	IM7	FC
PE/Fire™ 810 anti-mouse/human CD44	IM7	FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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