Direct-Blot HRP anti-6-His Epitope Tag Antibody anti-6-His Epitope Tag

Pricing & Availability

Clone: 6-His (See other available formats)
Regulatory Status: RUO
Other Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Isotype: Mouse IgG1, κ
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Product Citations: publications

6-His_DB-HRP_6-His_Epitope_Tag_Antibody_051118 — Western blot analysis of 15 µg cell lysates from HeLa cells transfected with (+) or without (−) His-tagged plasmid. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.05, 0.1 and 0.5 µ/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-His-HRP antibody, clone 6-His. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).

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906109

25 µL

90€

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906110

100 µL

272€

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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Epitope tag
Reported Reactivity: Species independent
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Purity: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.
RRID: AB_2734681 (BioLegend Cat. No. 906109)
AB_2734681 (BioLegend Cat. No. 906110)

Antigen Details

Biology Area: Cell Biology
Gene ID: NA
UniProt: View information about 6-His on UniProt.org

Related Products

Description	Clone	Applications

Other Formats

View All 6-His Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-6-His Epitope Tag Affinity Matrix	6-His	IP,Purification
Biotin anti-6-His Epitope Tag	6-His	WB,ICC,IP
FITC anti-6-His Epitope Tag	6-His	ICC
Purified anti-6-His Epitope Tag	6-His	WB,ICC,IP
Direct-Blot™ HRP anti-6-His Epitope Tag	6-His	WB
Alexa Fluor® 647 anti-6-His Epitope Tag	6-His	ICC

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Go To Top Version: 3 Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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