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ELISA MAX™ Deluxe Set Human ICAM-1

Pricing & Availability
Regulatory Status
RUO
Other Names
human Intercellular Adhesion Molecule 1, CD54, BB2, P3.58
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publications
ELISA-MAX_Deluxe-Set-Human-ICAM-1_121324
  • ELISA-MAX_Deluxe-Set-Human-ICAM-1_121324
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479704 5 plates 280€
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Description

A cell surface glycoprotein crucial in immune responses and inflammation. ICAM-1 is naturally expressed at low levels across immune, endothelial, and epithelial cells, but when faced with inflammatory stimuli, its presence is upregulated. This dynamic response allows ICAM-1 to serve as both an adhesion receptor and a signaling molecule, playing a vital role in recruiting leukocytes from the bloodstream to sites of inflammation, thereby influencing immune cell activity. Belonging to the immunoglobulin gene superfamily, ICAM-1 functions as a ligand for integrins like LFA-1 and Mac-1, facilitating critical interactions during immune responses. Its expression is influenced by a range of cytokines, such as IFN-γ and TNF-α, as well as factors like oxygen radicals and hypoxia. These interactions enable ICAM-1 to support immune cell trafficking, pathogen clearance, and T-cell activation. Elevated levels of soluble ICAM-1 (sICAM-1) have been observed in numerous inflammatory and malignant diseases, including cancer types like pancreatic carcinoma and malignant melanoma, and are associated with prognosis in conditions such as chronic lymphocytic leukemia and Hodgkin's disease. This increase in sICAM-1 is also seen in chronic conditions, including COPD, asthma, and cardiovascular diseases, underscoring ICAM-1's role in disease progression and prognosis. Through its multi-functional capabilities, ICAM-1 contributes to the body’s response to inflammation, the resolution of inflammatory processes, and even the regulation of tumor cell survival and spread. Understanding ICAM-1’s role in these processes opens doors for potential therapeutic strategies aimed at controlling inflammation and improving disease outcomes.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human ICAM-1 ELISA MAX™ Capture Antibody (200X)
  • Human ICAM-1 ELISA MAX™ Detection Antibody (200X)
  • Human ICAM-1 Lyophilized Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)
  • Assay Diluent D

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.07 ng/mL
Standard Range
0.23-15 ng/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4 
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered 
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions 
  • Timer

Antigen Details

Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Soluble Receptors
Gene ID
NA
UniProt
View information about ICAM-1 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 12.13.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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