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- Regulatory Status
- RUO
- Other Names
- Prf1, Pfp, Pfn, Pfn-1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 479504 | 5 plates | 300€ | ||||
Mouse perforin, encoded by the PRF1 gene, is a pore-forming protein found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. When these immune cells encounter a target cell, such as a virus-infected or cancerous cell, they release perforin, which then forms pores in the target cell’s membrane. This allows other molecules, like granzymes, to enter the target cell and induce apoptosis, effectively killing the harmful cell.
A mouse perforin molecule consists of three domains: a Membrane Attack Complex/Perforin (MACPF) domain at the N-terminus, a C2 domain which required Ca2+ ions responsible for membrane docking, and an Epidermal Growth Factor (EGF)-like domain; these domains are crucial for its pore-forming function on target cell membranes.
Perforin plays a crucial role in the immune system’s ability to eliminate infected or malignant cells and maintain overall immune regulation. Deficiencies in perforin can lead to severe immune disorders and increase susceptibility to infections and cancers.
BioLegend’s ELISA MAX™ Mouse Perforin Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse perforin. The set is designed for cost-effective and accurate quantification of mouse perforin in serum, plasma, and cell culture supernatant. BioLegend’s ELISA MAX™ Mouse Perforin Deluxe Sets are sensitive, accurate, and robust.
Kit Details
- Kit Contents
-
- Mouse Perforin Capture Antibody (200X)
- Mouse Perforin Detection Antibody (200X)
- Mouse Perforin Lyophilized Standard
- Avidin-HRP, 1000X
- Assay Diluent B (5X)
- Substrate Solution F
- Coating Buffer A (5X)
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1000 μL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
- Sensitivity
- 0.21 ± 0.08 ng/mL
- Standard Range
- 0.63 - 40 ng/mL
Antigen Details
- Cell Type
- NK cells, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 18646 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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