ELISA MAX™ Deluxe Set Mouse Perforin

Pricing & Availability
Regulatory Status
RUO
Other Names
Prf1, Pfp, Pfn, Pfn-1
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Product Citations
publications
ELISA-Max_Mouse-Perforin_1_031925
  • ELISA-Max_Mouse-Perforin_1_031925
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479504 5 plates 300€
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Description

Mouse perforin, encoded by the PRF1 gene, is a pore-forming protein found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. When these immune cells encounter a target cell, such as a virus-infected or cancerous cell, they release perforin, which then forms pores in the target cell’s membrane. This allows other molecules, like granzymes, to enter the target cell and induce apoptosis, effectively killing the harmful cell.

A mouse perforin molecule consists of three domains: a Membrane Attack Complex/Perforin (MACPF) domain at the N-terminus, a C2 domain which required Ca2+ ions responsible for membrane docking, and an Epidermal Growth Factor (EGF)-like domain; these domains are crucial for its pore-forming function on target cell membranes.

Perforin plays a crucial role in the immune system’s ability to eliminate infected or malignant cells and maintain overall immune regulation. Deficiencies in perforin can lead to severe immune disorders and increase susceptibility to infections and cancers.

BioLegend’s ELISA MAX™ Mouse Perforin Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse perforin. The set is designed for cost-effective and accurate quantification of mouse perforin in serum, plasma, and cell culture supernatant. BioLegend’s ELISA MAX™ Mouse Perforin Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse Perforin Capture Antibody (200X)
  • Mouse Perforin Detection Antibody (200X)
  • Mouse Perforin Lyophilized Standard
  • Avidin-HRP, 1000X
  • Assay Diluent B (5X) 
  • Substrate Solution F
  • Coating Buffer A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.21 ± 0.08 ng/mL
Standard Range
0.63 - 40 ng/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered 
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1000 μL
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions 
  • Timer

Antigen Details

Cell Type
NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
18646 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 03.19.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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