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FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

Pricing & Availability
Clone
UCHT1; HCD14; HIB19; 2H7; HCD56;
Regulatory Status
RUO
Isotype
Mouse IgG1, κ/Mouse IgG2b, κ
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Product Citations
publications
hlin2cocktail_fitc_1_083110
Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_1_083110
    Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_2_083110
    Human peripheral blood co-stained with CD11c APC and CD16 PE, gated on the lineage negative/dim and HLA-DR+ population
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348701 50 tests 465€
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Description

This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Wu X, et al. 2023. iScience. 26:106559. PubMed
  2. Ait Djebbara S, et al. 2023. Front Immunol. 14:1180900. PubMed
  3. Rosendahl Huber A, et al. 2022. STAR Protoc. 3:101361. PubMed
  4. Brandsma AM, et al. 2021. Blood Cancer Discov. 2:484. PubMed
  5. de Kanter JK, et al. 2021. Cell Stem Cell. 28:1726. PubMed
  6. Qian P et al. 2018. Cell stem cell. 22(5):740-754 . PubMed
  7. Brandsma AM, et al. 2021. Cancer Discov. . PubMed
  8. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  9. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  10. Awad MM, et al. 2021. Front Oncol. 11:696512. PubMed
  11. Webb K et al. 2018. Frontiers in immunology. 2.574305556 . PubMed
  12. Hasaart KAL, et al. 2022. iScience. 25:103736. PubMed
  13. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  14. Okumura T, et al. 2019. Stem Cell Res Ther. 10:185. PubMed
RRID
AB_10644012 (BioLegend Cat. No. 348701)

Antigen Details

Distribution

CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.

Cell Type
Dendritic cells, Eosinophils, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey.
2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551.
3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York.
4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955.
5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698.
6. Macdonald K, et al. 2002. Blood 100:4512.
7. Piccioli D, et al. 2007. Blood 109:5371.

Gene ID
915 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
UniProt
View information about Lineage on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.09.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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