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FITC anti-human MRP-14 (S100A9) Antibody

Pricing & Availability
Clone
MRP 1H9 (See other available formats)
Regulatory Status
RUO
Other Names
S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype
Mouse IgG1, κ
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Product Citations
publications
MRP-1H9_FITC_MRP-14_Antibody_1_101524
Human peripheral blood granulocytes surface stained with anti-human CD15 PerCP/Cyanine5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP-14 (clone MRP 1H9) FITC (left) or mouse IgG1, κ FITC isotype control (right)
  • MRP-1H9_FITC_MRP-14_Antibody_1_101524
    Human peripheral blood granulocytes surface stained with anti-human CD15 PerCP/Cyanine5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP-14 (clone MRP 1H9) FITC (left) or mouse IgG1, κ FITC isotype control (right)
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350703 25 tests 90€
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350704 100 tests 203€
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Description

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MRP-14
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone has been published as 1H9.

Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining  with paraffin-embedded tissue sections1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Robinson MJ, et al. 2002. J. Biol. Chem. 277:3658. (WB, IHC)
  2. Croce K, et al. 2009. Circulation 120:427. (IHC)
  3. Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
Product Citations
  1. Yoshida M, et al. 2022. Nature. 602:321. PubMed
RRID
AB_10662250 (BioLegend Cat. No. 350703)
AB_10662250 (BioLegend Cat. No. 350704)

Antigen Details

Structure
Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8
Distribution

MDSCs, neutrophils, monocyte, and ileal tissue eosinophils

Function
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction
Calcium
Cell Type
Eosinophils, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules
Antigen References

1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al.2006. J. Mol. Biol. 359:961.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176. 

Gene ID
6280 View all products for this Gene ID
UniProt
View information about MRP-14 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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