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FITC anti-human Podocalyxin Antibody

Pricing & Availability
Clone
mAb 84 (See other available formats)
Regulatory Status
RUO
Other Names
GCTM-2 antigen, Gp200, Podocalyxin-like protein 1, PC, PCLP-1, PODXL, PCLP, PCLP1, GCTM2
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
mAb84_FITC_Podocalyxin_Antibody_071918
Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and anti-human Podocalyxin (clone mAb 84, left) FITC or Mouse IgM, κ isotype control FITC (right).
  • mAb84_FITC_Podocalyxin_Antibody_071918
    Human teratocarcinoma cell line, NCCIT, stained with SSEA-4 APC and anti-human Podocalyxin (clone mAb 84, left) FITC or Mouse IgM, κ isotype control FITC (right).
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374303 25 tests 104€
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374304 100 tests 221€
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Description

Podocalyxin-like protein-1 (also known as PODXL, GP200, PCLP1 and GCTM2) is expressed on the surface of undifferentiated human ES cells and glomerular podocytes. PODXL was originally described as the main sialoprotein expressed by glomerular epithelial cells. Recently PODXL been associated with several forms of cancer and is being targeted as a possible therapeutic. Clone mAb 84 is reported to recognize and have cytotoxic affects against undifferentiated human ES cells. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats) include: WB, IP, cytotoxicity. The LEAF purified format is recommended for cytotoxicity assays. 

RRID
AB_2749881 (BioLegend Cat. No. 374303)
AB_2749881 (BioLegend Cat. No. 374304)

Antigen Details

Structure
Sialomucin family
Distribution

Stem cells, Glomerular podocytes

Function
Stem cell differentiation, regulatory factor
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References

1. Kerjaschki D, et al. 1984. J Cell Bio. 98:1591.
2. Horvat R, et al. 1986. J Cell Bio. 102:484.
3. Choo AB, et al. 2008. Stem Cells. 26:1454.
4. Tan HL, et al. 2009. Stem Cells. 27:1792.
5. Nielsen JS, et al. 2009. J Am Soc Nephrol. 20:1669.

Gene ID
5420 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org
Go To Top Version: 1    Revision Date: 07.19.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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