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- Clone
- KH114 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class I
- Isotype
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
FVB mouse splenocytes stained with KH114 FITC
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 115104 | 500 µg | 248€ | ||||
The KH114 antibody reacts with the H-2Kq MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kq haplotype. H-2Kq is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The KH114 antibody has not been reported to cross-react with other haplotypes.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- B10.Q mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1. Does not cross-react with other haplotypes (e.g. a, b, d, f, k, n, p, r, s, u, v).
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Application References
(PubMed link indicates BioLegend citation) -
- Hasendrug K, et al. 1987. Immunogenetics 25:136.
- Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
- Nanni P, et al. 2001. J. Exp. Med. 194:1195.
- Product Citations
-
- RRID
-
AB_313625 (BioLegend Cat. No. 115104)
Antigen Details
- Structure
- MHC class I
- Distribution
-
Nucleated cells of the H-2Kq haplotype
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
4. Hasendrug K, et al. 1987. Immunogenetics 25:136. - Gene ID
- 14972 View all products for this Gene ID
- UniProt
- View information about H-2Kq on UniProt.org
Other Formats
View All H-2Kq Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse H-2Kq | KH114 | FC |
| Purified anti-mouse H-2Kq | KH114 | FC |
| Alexa Fluor® 647 anti-mouse H-2Kq | KH114 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse H-2Kq
FVB mouse splenocytes stained with KH114 FITC -
Purified anti-mouse H-2Kq
FVB mouse splenocytes stained with purified KH114, followed ... -
Alexa Fluor® 647 anti-mouse H-2Kq
FVB mouse splenocytes stained with purified KH114 Alexa Fluo...
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