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FITC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
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Product Citations
publications
SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
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405201 100 µg 34€
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405202 500 µg 95€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
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  5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
  6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed
Product Citations
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  17. Yang M 2012. J Virol. 86:7072. PubMed
  18. Pan H, et al. 2020. Mol Psychiatry. . PubMed
  19. Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed
  20. Sánchez-Salguero E, et al. 2021. Front Immunol. 12:712130. PubMed
  21. Hu Y, et al. 2022. Nat Commun. 13:5313. PubMed
  22. Li S, et al. 2016. PLoS One. 11: 0163893. PubMed
  23. Lee J, et al. 2014. J Rheumatol. 41:2425. PubMed
  24. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
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  30. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  31. Schmutz S, et al. 2009. J Immunol. 183:2217. PubMed
  32. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  33. Cheng LS, et al. 2022. J Transl Med. 20:415. PubMed
  34. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
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  38. Oda Y, et al. 2021. Sci Adv. 7:eabj6895. PubMed
  39. Li X, et al. 2021. Oncol Lett. 22:788. PubMed
  40. Cho H, et al. 2009. Immunol Lett. 127:39. PubMed
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Go To Top Version: 5    Revision Date: 06.17.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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