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- Clone
- 29F.1A12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PD-1, Programmed Death-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- PD-1 cDNA followed by PD-1-Ig fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
-
GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3 and in vivo blocking of PD-1 binding to its ligands2,3. -
Application References
(PubMed link indicates BioLegend citation) -
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Lßzßr-Molnßr E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
- Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
- Product Citations
-
- RRID
-
AB_2616834 (BioLegend Cat. No. 135236)
AB_2616834 (BioLegend Cat. No. 135238)
AB_2616834 (BioLegend Cat. No. 135234)
AB_2616834 (BioLegend Cat. No. 135237)
AB_2616834 (BioLegend Cat. No. 135235)
AB_2616834 (BioLegend Cat. No. 135233)
Antigen Details
- Structure
- A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
- Distribution
-
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
- Function
- Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
- Ligand/Receptor
- B7-H1 (PD-L1) and B7-DC (PD-L2)
- Cell Type
- B cells, T cells
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313 - Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All CD279 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated (3 days) C57BL/6 splenocytes stain... 
Con A stimulated (3 days) C57BL/6 splenocytes stained with r... -
Purified anti-mouse CD279 (PD-1)
Con A and IL-2 stimulated mouse splenocytes (day 3) were sta... 
Fresh, frozen mouse spleen was stained with purified CD279 c... -
PerCP/Cyanine5.5 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) stain... -
APC anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) stain... -
Biotin anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated (3 days) C57BL/6 splenocytes stain... 
Con A-stimulated (3 days) C57BL/6 splenocytes stained with C... -
FITC anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days)... 
-
PE/Cyanine7 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated (3 days) C57BL/6 mouse splenocytes... -
Brilliant Violet 421™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were ... 
Mice were injected subcutaneously with sheep red blood cells... -
Brilliant Violet 605™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were ... -
APC/Cyanine7 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated splenocytes (3 days) were stained ... -
Brilliant Violet 785™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were ... 
-
PE/Dazzle™ 594 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... 
-
Alexa Fluor® 647 anti-mouse CD279 (PD-1)
Con A and IL-2 stimulated C57BL/6 splenocytes (three days) w... 
-
Brilliant Violet 711™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... 
-
GoInVivo™ Purified anti-mouse CD279 (PD-1)
-
APC/Fire™ 750 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days)... 
-
Brilliant Violet 510™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days)... 
-
Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)
Con A and IL-2 stimulated mouse splenocytes (day 3) were sta... -
APC/Fire™ 810 anti-mouse CD279 (PD-1) Antibody
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
PE/Fire™ 810 anti-mouse CD279 (PD-1) Antibody
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (three d... -
PE/Cyanine5 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) were ... -
PE/Fire™ 640 anti-mouse CD279 (PD-1)
C57BL/6 mouse splenocytes were stimulated with Con-A and IL-... -
Spark Red™ 718 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57 splenocytes (3 days) were stai... -
PerCP/Fire™ 806 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
Brilliant Violet 750™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (3 days) stain... -
PerCP/Fire™ 780 anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
PE/Fire™ 700 anti-mouse CD279 (PD-1)
Unstimulated BALB/c splenocytes were stained with anti-mouse... 
Con A and IL-2 stimulated BALB/c splenocytes (three days) we... -
Brilliant Violet 650™ anti-mouse CD279 (PD-1)
Con-A and IL-2 stimulated C57BL/6 mouse splenocytes (3 days)... -
Spark Blue™ 574 anti-mouse CD279 (PD-1) (Flexi-Fluor™)
-
Spark PLUS B550™ anti-mouse CD279 (PD-1)
Con A+IL-2 stimulated BALB/c splenocytes (3 days) were stain...
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