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- Clone
- B122 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- E. coli -expressed, recombinant mouse IL-1β
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
ELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1β recombinant protein ranging from 2000 to 30 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8. -
Application References
(PubMed link indicates BioLegend citation) -
- Hogquist K, et al. 1991. J. Immunol. 146:1534.
- Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut)
- Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture)
- Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011.
- Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion)
- Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC)
- Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB)
- Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed
- RRID
-
AB_2888752 (BioLegend Cat. No. 503510)
AB_2888752 (BioLegend Cat. No. 503512)
AB_2888752 (BioLegend Cat. No. 503508)
AB_2888752 (BioLegend Cat. No. 503511)
AB_2888752 (BioLegend Cat. No. 503509)
AB_2888752 (BioLegend Cat. No. 503507)
Antigen Details
- Structure
- Cytokine; 17.5 kD (Mammalian)
- Bioactivity
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
- Cell Targets
- B cells, T cells, monocytes
- Receptors
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095. - Regulation
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 16176 View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All IL-1β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse / rat IL-1β | B122 | ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB |
| Biotin anti-mouse / rat IL-1β | B122 | ELISA |
| Ultra-LEAF™ Purified anti-mouse / rat IL-1β | B122 | ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB |
| GoInVivo™ Purified anti-mouse/rat IL-1β | B122 | ELISA Capture,ELISPOT Capture,Depletion,IHC-F,IP,Neut,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse / rat IL-1β
-
Biotin anti-mouse / rat IL-1β
-
Ultra-LEAF™ Purified anti-mouse / rat IL-1β
-
GoInVivo™ Purified anti-mouse/rat IL-1β
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