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LEAF™ Purified anti-mouse IL-18BP Antibody

Pricing & Availability
Clone
Poly23A31 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-18-Binding Protein, Tadekinig-Alfa
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly23A31_PURE_IL-18BP_Block_040425
Recombinant mouse IL-18BP-Fc chimera (Cat. No. 792304) (black circles) binds to immobilized mouse IL-18 (Cat. No. 767006). LEAF™ purified anti-mouse IL-18BP (Poly23A31) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified polyclonal goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 0.6 µg/mL mouse IL-18BP-Fc chimera to 2 µg/mL immobilized mouse IL-18. ND50 range: 1.5 - 8 µg/mL.
  • Poly23A31_PURE_IL-18BP_Block_040425
    Recombinant mouse IL-18BP-Fc chimera (Cat. No. 792304) (black circles) binds to immobilized mouse IL-18 (Cat. No. 767006). LEAF™ purified anti-mouse IL-18BP (Poly23A31) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified polyclonal goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 0.6 µg/mL mouse IL-18BP-Fc chimera to 2 µg/mL immobilized mouse IL-18. ND50 range: 1.5 - 8 µg/mL.
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538204 100 µg 280€
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Description

As the name suggests, IL-18 binding protein (IL-18BP) is a binding protein that binds withhigh affinity (Kd = 400 pM) to IL-18 in a stoichiometric ratio to neutralize IL-18 activity.  IL-18BP also interacts with IL-37 at concentrations of 50- to 100-fold molar higher than IL-37. In an in-vitro NK-cell line culture, this interaction increases the inhibitory effect of IL-18BP on IL-18-induced IFN-γ. IL-18BP knockout (KO) mice demonstrates reduced abundance of splenic NK cells, increase in immature NK cells, and a decrease in mature NK cells. The changes in NK cells are associated with a decrease in circulating plasma IL-18. IL-18 and IL-18BP are elevated in patients with eczema; also, IL-18 and IL-18BP are high in serum of patients with chronic liver disease. In addition, patients with heart failure have shown increase in IL-18 but decrease in IL-18BP levels. Currently, IL-18BP is in a clinical trial for the treatment of adult-onset Still’s disease, an inflammatory disease, which is characterized by high levels of IL-18 in plasma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IL-18BP-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/μg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the ability to block the binding of a spike concentration of mouse IL-18BP-Fc chimera (Cat. No. 792304) to immobilized mouse IL-18 (Cat. No. 767006). The ND50 range for this effect is 1.5 - 8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Dimer
Distribution

Mononuclear cells, leukocytes, keratinocytes, dermal fibroblasts. IFN-γ induces IL-18BP in colon carcinoma cells, ovarian epithelial cancer cells, HepG2 cells, HaCaT keratinocyte, and fibroblast-like synovial cells.

Function
Downregulates Th1 responses by binding to IL-18. Controls the cell number and function of NK cells and maintains the levels of circulating IL-18. Regulated by LXR (transcription factor), IL-27, and IFN-γ. Induced by IL-12 in monocytes via IFN-γ.
Interaction
IL-37
Ligand/Receptor
IL-18
Cell Type
Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Paulukat J, et al. 2001. J Immunol. 167:7038-43.
  2. Veenstra KG, et al. 2002. J Immunology. 168:22282-7.
  3. Bufler P, et al. 2002. Proc Natl Acad Sci USA. 99:13723-8.
  4. Mallat Z, et al. 2004. FASEB. 18:1752-4.
  5. Wittmann M, et al. 2012. PLoS One. 7:e38751.
  6. Dinarello CA, et al. 2013. Front Immunol. 4:289.
  7. Carbotti G, et al. 2013. Clin Cancer Res. 19:4611-20.
  8. Pourcet B, et al. 2016. Sci Rep. 6:25481.
  9. Hu Y, et al. 2017. Mediators Inflamm. 2017:3090782.
  10. Harms RZ, et al. 2017. Front Immunol. 8:1020.
Gene ID
NA
UniProt
View information about IL-18BP on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 04.04.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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