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- Regulatory Status
- RUO
- Other Names
- PAI-1, Insulin, Leptin, Cortisol
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The Human Diabesity Panel is a multiplex bead-based assay panel, which uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows for the simultaneous quantification of 11 human proteins involved in diabesity, including PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol, IL-1β, IL-6 and GLP-1 (Active). This assay panel provides broader dynamic ranges than traditional ELISA methods and has been validated for use with tissue culture supernatant, serum, and EDTA plasma samples.
The LEGENDplex™ Human Diabesity Panel is configured as shown below depending on sample types and required dilutions:
|
Catalog No. |
Plex Size |
Targets |
Recommended Sample Type |
Recommended Dilution Factor |
|
740909 740910 |
11-plex |
PAI-1, GLP-1 (Total), Insulin, C-Peptide, TNFα, Glucagon, Leptin, Cortisol*, IL-1β, IL-6 and GLP-1 (Active) |
Tissue Culture |
Varies |
|
740911 740912 |
7-plex |
GLP-1 (Total), C-Peptide, TNFα, Glucagon, IL-1β, IL-6 and GLP-1 (Active) |
Serum, Plasma |
1:1 |
|
740913 740914 |
4-plex |
PAI-1, Insulin, Leptin, Cortisol* |
Serum, Plasma |
1:8 |
* This panel introduces a competitive assay for Cortisol (on B5 bead). A concentrated cortisol tracer is provided in this kit which will need to be diluted 100-fold before the assay. This cortisol tracer will be added to all wells before adding the beads. The competition between the cortisol tracer and the standard cortisol will characterize the standard curve. Due to the nature of the assay, the standard curve will have the highest signal at the lowest standard cortisol concentration and will decrease as the standard cortisol concentration increases.
Note: The cortisol tracer is for the cortisol assay ONLY and does not affect any other target.
The LEGENDplex™ Human Diabesity Panel is designed to allow flexible customization within the panel. Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
This assay is for research use only.
Kit Contents
- Kit Contents
-
- Setup Beads: PE Beads
- Setup Beads: Raw Beads
- Capture Beads
- Detection Antibodies
- Standard Cocktail, Lyophilized
- Human Cortisol Tracer, 100X
- SA-PE
- Assay Buffer
- Matrix B, Lyophilized
- Wash Buffer, 20X
- Plate Sealers
- Filter Plate
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Micro FACS tubes (if the flow cytometer does not contain an autosampler)
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Manual
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines, Enzymes and Regulators
- Gene ID
- 5054 View all products for this Gene ID 3630 View all products for this Gene ID 3952 View all products for this Gene ID
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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