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Pacific Blue™ anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
W6D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W6D3_PB_102809
Human peripheral blood granulocytes stained with W6D3 Pacific Blue™.
  • W6D3_PB_102809
    Human peripheral blood granulocytes stained with W6D3 Pacific Blue™.
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
323021 25 tests 113€
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323022 100 tests 235€
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323024 100 µg 235€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per 106 cells in 100 µl volume.
For µg size, the suggested use of this reagent for immunofluorescent staining is ≤1.0 µg per 106 cells in 100 µl volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Silvin A, et al. 2020. Cell. 182:1401. PubMed
  2. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  3. Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed
  4. Girard P, et al. 2022. Front Bioeng Biotechnol. 10:895735. PubMed
  5. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  6. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  7. Køllgaard T, et al. 2015. PLoS One. 10: 0131934. PubMed
  8. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  9. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  10. Iversen T, et al. 2014. Clin Cancer Res. 20:221. PubMed
RRID
AB_2105359 (BioLegend Cat. No. 323021)
AB_2105359 (BioLegend Cat. No. 323022)
AB_2105359 (BioLegend Cat. No. 323024)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Other Formats

View All CD15 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) W6D3 FC,CyTOF®
FITC anti-human CD15 (SSEA-1) W6D3 FC
PE anti-human CD15 (SSEA-1) W6D3 FC
APC anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine5 anti-human CD15 (SSEA-1) W6D3 FC
PerCP anti-human CD15 (SSEA-1) W6D3 FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1) W6D3 FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready) W6D3 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1) W6D3 FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 750 anti-human CD15 (SSEA-1) W6D3 FC
FITC anti-human CD15 (SSEA-1) W6D3 FC
Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1) W6D3 PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1) W6D3 FC
APC/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1) W6D3 PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1) W6D3 PG
PE anti-human CD15 (SSEA-1) W6D3 FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody W6D3 FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1) W6D3 PG
GMP FITC anti-human CD15 (SSEA-1) W6D3 FC
Spark YG™ 593 anti-human CD15 (SSEA-1) W6D3 FC
GMP PE anti-human CD15 (SSEA-1) W6D3 FC
GMP Pacific Blue™ anti-human CD15 (SSEA-1) W6D3 FC
Spark Violet™ 500 anti-human CD15 (SSEA-1) W6D3 FC
Spark UV™ 387 anti-human CD15 (SSEA-1) W6D3 FC
PE/Cyanine7 anti-human CD15 (SSEA-1) W6D3 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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