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Pacific Blue™ anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
390_PB_062310
C57BL/6 mouse splenocytes stained with 390 Pacific Blue™
  • 390_PB_062310
    C57BL/6 mouse splenocytes stained with 390 Pacific Blue™
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102421 25 µg 100€
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102422 100 µg 287€
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  2. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  3. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  4. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  5. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  6. Svec J, et al. 2022. Cancers (Basel). 14:. PubMed
  7. Reinitz F, et al. 2022. Elife. 11:. PubMed
  8. Skowron K, et al. 2016. Sci Rep. 6:35854. PubMed
  9. Scheele C, et al. 2017. Nature. 542:313-317. PubMed
  10. Vesprey A, et al. 2021. J Bone Miner Res. 36:1000. PubMed
  11. Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed
  12. Lee C, et al. 2014. Cancer Res. 74:341. PubMed
  13. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  14. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  15. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  16. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  17. Zhao X, et al. 2020. Methods Mol Biol. 2346:21. PubMed
  18. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  19. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  20. Farsaci B, et al. 2014. Cancer Immunol Res. 2:1090. PubMed
  21. Frech S, et al. 2021. J Invest Dermatol. . PubMed
  22. Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed
  23. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  24. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  25. An X, et al. 2020. Thyroid. . PubMed
  26. Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  27. Xie X, et al. 2018. FASEB J. 32:1296. PubMed
  28. Peloquin GL, et al. 2019. Respir Res. 20:123. PubMed
  29. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  30. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  31. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  32. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  33. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  34. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_10613457 (BioLegend Cat. No. 102421)
AB_10613457 (BioLegend Cat. No. 102422)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 2    Revision Date: 07.19.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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