PE anti-human CD55 Antibody anti-CD55

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Clone: JS11 (See other available formats)
Regulatory Status: RUO
Workshop: VI N-L060
Other Names: DAF (Decay Accelerating Factor)
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes stained with JS11 PE, then detected with anti-mouse IgG FITC

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311308

100 tests

215€

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Description

CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_314865 (BioLegend Cat. No. 311308)

Antigen Details

Structure: GPI-achored regulator of complement activation (RCA), type I membrane glycoprotein, 60-70 kD
Distribution: Hematopoietic cells including erythrocytes and many non-hematopoietic cells
Function: Protects against inappropriate complement activation and cell damage, induces signal transduction, reduces the efficiency of NK cell lysis
Ligand/Receptor: C3b, C4b, CD97, Coxsackie viruses
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221
Gene ID: 1604 View all products for this Gene ID
UniProt: View information about CD55 on UniProt.org

Documentation

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Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD55 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD55	JS11	FC
Biotin anti-human CD55	JS11	FC
FITC anti-human CD55	JS11	FC
PE anti-human CD55	JS11	FC
Purified anti-human CD55	JS11	FC
PE/Cyanine7 anti-human CD55	JS11	FC
PerCP/Cyanine5.5 anti-human CD55	JS11	FC
TotalSeq™-A0383 anti-human CD55	JS11	PG
TotalSeq™-C0383 anti-human CD55	JS11	PG
TotalSeq™-B0383 anti-human CD55	JS11	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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