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- Clone
- W16032B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with PE EOS (clone W16032B) (left) or rat IgG2a, κ isotype PE (right), and FOXP3 (clone 206D) Alexa Fluor® 647. Dot plots are gated on CD3+CD4+ cells.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 397403 | 25 tests | 123€ | ||||
| 397404 | 100 tests | 317€ | ||||
EOS also known as IKZF4 is an IKAROS Family Member expressed in lymphocytes, specifically in Tregs. It plays an important role in the inhibitory function of Tregs. It may be involved in the development of the central and peripheral nervous systems as well as lymphoid development.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human EOS recombinant protein (271-520 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2832773 (BioLegend Cat. No. 397403)
AB_2832773 (BioLegend Cat. No. 397404)
Antigen Details
- Structure
- IKAROS family member
- Distribution
-
Lymphocytes, specifically in Tregs
- Function
- Essential for inhibitory function of Tregs
- Interaction
- Binds to 5'GGGAATRCC-3' Ikaros-binding sequence
- Antigen References
-
- Honma Y, et al. 1999. FEBS Lett. 447:76-80.
- Perdomo J, et al. 2000. J. Biol. Chem. 275:38347-54.
- Westman BJ, et al. 2004. Biochemistry. 43:13318-27.
- Pan F, et al. 2009. Science. 10.1126/science.1176077.
- Gene ID
- 64375 View all products for this Gene ID
- UniProt
- View information about EOS on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All EOS Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human EOS | W16032B | ICFC |
| PE anti-human EOS | W16032B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human EOS
Human peripheral blood lymphocytes were surface stained with... -
PE anti-human EOS
Human peripheral blood lymphocytes were surface stained with...
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