PE anti-mouse CD107b Mac-3 Antibody anti-Mac-3

Pricing & Availability

Clone: M3/84 (See other available formats)
Regulatory Status: RUO
Other Names: CD107b, LAMP-2
Isotype: Rat IgG1, κ
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Product Citations: publications

Thioglycolate-elicited peritoneal macrophages stained with M3/84 PE

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108505

50 µg

98€

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108506

200 µg

267€

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Description

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1-4 and immunohistochemical staining of acetone-fixed frozen sections^5,6and paraformaldehyde-fixed paraffin-embedded sections^9-11.
RRID: AB_313386 (BioLegend Cat. No. 108505)
AB_313386 (BioLegend Cat. No. 108506)

Antigen Details

Structure: 110 kD
Distribution: Macrophages, some myeloid cell lines
Function: Differentiation and activation
Cell Type: Macrophages
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.
Gene ID: 16784 View all products for this Gene ID
UniProt: View information about CD107b on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD107b Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD107b (Mac-3)	M3/84	FC
PE anti-mouse CD107b (Mac-3)	M3/84	FC
Purified anti-mouse CD107b (Mac-3)	M3/84	FC,IP,ICC,IHC-P,IHC-F
Biotin anti-mouse CD107b (Mac-3)	M3/84	FC
Alexa Fluor® 488 anti-mouse CD107b (Mac-3)	M3/84	FC
Alexa Fluor® 647 anti-mouse CD107b (Mac-3)	M3/84	FC,ICC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 10.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

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