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PE anti-mouse CD137 Antibody

Pricing & Availability
Clone
17B5 (See other available formats)
Regulatory Status
RUO
Other Names
4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CD137
Isotype
Syrian Hamster IgG
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Product Citations
publications
17b5
Con A-stimulated (3 days) splenocytes stained with 17B5 PE
  • 17b5
    Con A-stimulated (3 days) splenocytes stained with 17B5 PE
Compare all formats See PE spectral data
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106105 50 µg 84€
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106106 200 µg 254€
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Description

CD137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CD137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CD137 with its ligand, 4-1BBL (CD137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106108).

Application References

(PubMed link indicates BioLegend citation)
  1. Lee SW, et al. 2005. J. Immunol. 174:6803. (FC)
  2. Zheng G, et al. 2004. J. Immunol. 173:2428. (Block)
  3. Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed
  4. Nishimoto H, et al. 2005. Blood 106:4241. (FC)
  5. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  2. Dúcka M, et al. 2021. Neoplasia. 23:326. PubMed
  3. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  4. Shitaoka K, et al. 2018. Cancer Immunol Res. 6:378. PubMed
  5. Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed
  6. Toomer G, et al. 2022. Viruses. 14:. PubMed
  7. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  8. Marangoni F, et al. 2021. Cell. . PubMed
  9. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  10. Meadows H 2005. J Leukoc Biol. 78:1070. PubMed
  11. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  12. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  13. Pérez-Guijarro E, et al. 2020. Nat Med. 26:781. PubMed
  14. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Németh T, et al. 2014. Fungal Genet Biol. 65:48. PubMed
  16. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
RRID
AB_2287565 (BioLegend Cat. No. 106105)
AB_2287565 (BioLegend Cat. No. 106106)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
TNF-R superfamily, 39 kD
Distribution

Activated T cells and B cells

Function
T-B cell costimulation
Ligand/Receptor
4-1BB ligand (CDw137L)
Cell Type
B cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
21942 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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