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PE anti-mouse CD40 Antibody

Pricing & Availability
Clone
3/23 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
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Product Citations
publications
3slash23_PE_081908.jpg
BALB/c splenocytes stained with 3/23 PE
  • 3slash23_PE_081908.jpg
    BALB/c splenocytes stained with 3/23 PE
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Cat # Size Price Quantity Check Availability Save
124609 25 µg 72€
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124610 100 µg 220€
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Description

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD40 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
  2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations
  1. Liu J, et al. 2019. Immunity. 50:600. PubMed
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  11. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
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  13. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  14. Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed
  15. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  16. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  17. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  18. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  19. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  20. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  21. Jing L, et al. 2022. Front Immunol. 13:864995. PubMed
  22. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  23. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  24. Ding M, et al. 2019. Exp Ther Med. 18:2323. PubMed
  25. Wang J, et al. 2017. Mol Med Rep. 16:8434. PubMed
  26. Ise W, et al. 2018. Immunity. 48:702. PubMed
  27. Ozeri E, et al. 2012. J Immunol. 189:146. PubMed
  28. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  29. Wang Y, et al. 2009. Clin Cancer Res. 5.417361111. PubMed
  30. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  31. Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
  32. Mao L, et al. 2022. Front Immunol. 13:833418. PubMed
  33. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  34. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  35. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  36. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  37. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  38. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  39. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  40. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
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  47. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  48. Wallner S, et al. 2013. PLoS One. 8:65178. PubMed
  49. Liang Z, et al. 2022. iScience. 25:105233. PubMed
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  51. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
  52. Shi GN, et al. 2021. Ther Adv Med Oncol. 13:1758835920987056. PubMed
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RRID
AB_1134075 (BioLegend Cat. No. 124609)
AB_1134075 (BioLegend Cat. No. 124610)

Antigen Details

Structure
48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution

Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.

Function
Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand/Receptor
CD154 on T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.

Gene ID
21939 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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