PE anti-mouse CD69 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse dendritic epidermal T cell line Y245

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation^1-3, immunohistochemistry^4,5, and immunoprecipitation¹.

This antibody has been characterized in the literature as containing a lambda light chain.

Application References

(PubMed link indicates BioLegend citation)

1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
2. Sobel ES, et al. 1993. J. Immunol. 150:673.
3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
7. Lee JW, et al. 2006. Nature Immunol. 8:181.
8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
9. Jordan JM, et al. 2008. 76:3717. PubMed
10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed

Product Citations

1. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
2. Chen N, et al. 2021. Front Immunol. 12:756423. PubMed
3. Shenoy AT, et al. 2022. Cytometry A. 101:892. PubMed
4. Li X, et al. 2022. J Clin Invest. 132: . PubMed
5. Huis In 't Veld LGM, et al. 2022. Cell Mol Life Sci. 79:231. PubMed
6. Xie T, et al. 2022. Mol Med Rep. 25: . PubMed
7. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
8. Kang IH, et al. 2022. iScience. 25:104818. PubMed
9. Liu M, et al. 2022. Cells. 11: . PubMed
10. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
11. Merk VM, et al. 2023. Genes Immun. 24:52. PubMed
12. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
13. Yuan Z, et al. 2011. Vaccine. 29:6614. PubMed
14. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
15. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
16. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
17. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
18. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
19. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
20. Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed
21. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
22. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
23. Bian L, et al. 2022. Front Immunol. 13:938598. PubMed
24. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
25. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
26. Alotaibi F, et al. 2021. Front Immunol. 11:584937. PubMed
27. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
28. Mier-Aguilar C, et al. 2016. PLoS One. 11:e0168155. PubMed
29. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
30. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
31. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
32. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
33. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
34. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
35. Holan V, et al. 2010. J Immunol. 184:2124. PubMed
36. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
37. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
38. Artinger K, et al. 2015. PLoS One. 10: 0135087. PubMed
39. Velázquez K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G699 - G712. PubMed
40. Hu J, et al. 2020. Biomed Pharmacother. 127:110229. PubMed
41. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
42. Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
43. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
44. Huang Z, et al. 2012. J Immunol. 188:5867. PubMed
45. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
46. Suryawanshi RK, et al. 2021. Nat Commun. 12:6020. PubMed
47. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
48. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
49. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
50. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
51. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
52. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
53. Watanabe M, et al. 2020. Nat Commun. 4.808333333. PubMed
54. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
55. Place D, et al. 2017. PLoS One. 10.1371/journal.pone.0190384. PubMed
56. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
57. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
58. Kemp V, et al. 2018. Cancer Gene Ther. 26:268. PubMed
59. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
60. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
61. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
62. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
63. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
64. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
65. Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed
66. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
67. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
68. X X, et al. 2016. J Immunol . 197: 1683-1691. PubMed
69. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
70. Yan Q, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.05.006. PubMed
71. Skadow M, et al. 2019. J Immunol. 203:370. PubMed
72. van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed
73. Passos C, et al. 2014. Food Chem. 15:161. PubMed
74. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
75. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
76. Lopes N, et al. 2022. Elife. 11:. PubMed
77. Jang A, et al. 2015. Life Sci . 135: 138-146. PubMed
78. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
79. Demaria O, et al. 2022. Cell Rep Med. 3:100783. PubMed
80. Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed
81. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
82. Guo X, et al. 2020. J Cell Mol Med. . PubMed
83. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
84. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
85. Amantini C, et al. 2017. Oncotarget. 8:90766. PubMed
86. Yang FM, et al. 2022. Front Immunol. 13:918241. PubMed
87. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
88. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
89. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
90. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
91. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
92. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed

See More

RRID

AB_313110 (BioLegend Cat. No. 104507)
AB_313110 (BioLegend Cat. No. 104508)

Antigen Details

Structure

C-type lectin, 27/33 kD

Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function

Lymphocyte activation

Cell Type

B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs

Biology Area

Costimulatory Molecules, Immunology, Innate Immunity

Molecular Family

CD Molecules

Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID

12515 View all products for this Gene ID

UniProt

View information about CD69 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis