- Clone
- TY/23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- 5'-nucleotidase, Nt5e, 5'-NT, Ecto-5'-nucleotidase
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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117203 | 25 µg | 88€ | ||||
117204 | 100 µg | 181€ |
CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- BALB/c mouse splenocytes and CHO cells transfected with the mouse CD73 gene, Nt5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
The TY/23 antibody has been reported to inhibit the enzymatic activity of CD73.
-
Application References
(PubMed link indicates BioLegend citation) -
1. Zhang B. 2010. Cancer Res. 70:6407-11.
2. Stagg J, et al. 2010. Proc Natl Acad Sci U S A. 107:1547-52.
3. Forte G, et al. 2012. J Immunol. 189:2226-33. - RRID
-
AB_3068054 (BioLegend Cat. No. 117203)
AB_3068054 (BioLegend Cat. No. 117204)
Antigen Details
- Structure
- 69 kD GPI-anchored surface protein
- Distribution
-
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
- Function
- A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
- Ligand/Receptor
- Nucleoside 5' monophosphate
- Cell Type
- B cells, Mesenchymal Stem Cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
- Yamashita Y, et al. 1998. Eur J Immunol. 28:2981-90.
- Resta R, et al. 1998. Immunol Rev. 161:95-109.
- Gene ID
- 23959 View all products for this Gene ID
- UniProt
- View information about CD73 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD73 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD73 | TY/23 | FC |
PE anti-mouse CD73 | TY/23 | FC |
Brilliant Violet 605™ anti-mouse CD73 | TY/23 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD73
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ... -
PE anti-mouse CD73
C57BL/6 mouse splenocytes were stained with anti-mouse CD3&e... -
Brilliant Violet 605™ anti-mouse CD73
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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