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PE anti-mouse CD80 Antibody

Pricing & Availability
Clone
W17200C (See other available formats)
Regulatory Status
RUO
Other Names
B-Lymphocyte activation antigen B7.1, CD80, Ly53, TSA1, CD28 ligand (CD28L), MIC17
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17200C_PE_CD80_Antibody_1_010825
LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 and anti-mouse CD80 (clone W17200C) PE (left) or rat IgG2a, κ PE isotype control (right).
  • W17200C_PE_CD80_Antibody_1_010825
    LPS-stimulated (3 days) C57BL/6 mouse splenocytes were stained with anti-mouse/human CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 and anti-mouse CD80 (clone W17200C) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
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600055 25 µg 142€
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600056 100 µg 308€
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Description

The B7 family of proteins are structurally related, cell membrane-associated molecules that regulate immune responses by delivering costimulatory or coinhibitory signals via their ligands. So far, eight family members have been identified, including B7.1 (CD80), B7.2, B7-H1, B7-H2, B7-H4, B7-H6, PD-L1, and PD-L2. These proteins are mainly expressed by activated antigen presenting cells (APCs) and when paired with either CD28 or CTLA-4 on a T lymphocyte, they induce an increase or decrease in the downstream signaling activity of the MHC-TCR interaction between APCs and T cells. B7.1 has been shown to bind both CTLA-4 and CD28. However, it has a 20-fold higher affinity to CTLA-4 than to CD28. Despite their structural similarities, the individual contribution of B7.1 and B7.2 to the development of pathogenic T cells in autoimmune diseases and protective T cells in infectious diseases is markedly distinct. Besides regulating T cell immune responses, B7 also plays a role in anti-tumor immunity. This is confirmed by demostrating that cytotoxic T cells were able to eradicate tumor cells transfected with B7.1 and B7.2. After activation, follicular lymphoma was shown to upregulate B7.1 and other costimulatory and adhesion molecules, therefore increasing APC activity and amplifying primed T cell responses. Homozygous null B7.1/B7.2 mice fail to generate antigen specific IgG1 and IgG2a responses, suggesting that B7 proteins are important in Ig immune responses. Mouse and human B7.1 share 44% amino acid identity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse B7.1 (CD80)-Fc Chimera
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Antigen Details

Structure
Homodimer
Distribution

Activated B cells, monocytes, macrophages, dendritic cells

Function
Plays a role in T cell activation; CD80 and CD86 play an important role in T cell priming
Interaction
T cells
Ligand/Receptor
CD28, CTLA-4
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Freeman GJ, et al. 1993. Science. 262:909-11. 
  2. Azuma M, et al. 1993. Nature. 366:76-9. 
  3. Gimmi CD, et al. 1991. Proc Natl Acad Sci USA. 88:6575-9. 
  4. Linsley PS, et al. 1991. J Exp Med. 173:721-30. 
  5. Greaves P and Gribben JG. 2013. Blood. 121: 734-44. 
  6. Czuczman MS, et al. 2005. J Clin Oncol. 23:4390-8.
Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01.08.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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