PE anti-mouse/human CD15 SSEA-1 Antibody anti-SSEA-1

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Clone: MC-480 (See other available formats)
Regulatory Status: RUO
Other Names: Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Isotype: Mouse IgM, κ
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Product Citations: publications

F9 (mouse embryonic carcinoma cell line) stained with MC-480 PE

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125605

25 tests

123€

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125606

100 tests

220€

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Description

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, and immunohistochemistry¹ of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
RRID: AB_1089193 (BioLegend Cat. No. 125605)
AB_1089193 (BioLegend Cat. No. 125606)

Antigen Details

Structure: Carbohydrate epitope, Lewis blood group antigens
Distribution: Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells
Function: Adhesion, differentiation
Cell Type: Embryonic Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.
Gene ID: 14345 View all products for this Gene ID 2526 View all products for this Gene ID
UniProt: View information about CD15 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD15 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse/human CD15 (SSEA-1)	MC-480	FC
Biotin anti-mouse/human CD15 (SSEA-1)	MC-480	FC
Purified anti-mouse/human CD15 (SSEA-1)	MC-480	FC,IP,WB,IHC-F,IHC-P
Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1)	MC-480	FC
Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1)	MC-480	FC
FITC anti-mouse/human CD15 (SSEA-1)	MC-480	FC
TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1)	MC-480	PG
APC anti-mouse/human CD15 (SSEA-1)	MC-480	FC
TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1)	MC-480	PG
TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1)	MC-480	PG
TotalSeq™-Bn0076 anti-mouse/human CD15 (SSEA-1)	MC-480	SB

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

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