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PE anti-mouse IFN-γR β chain Antibody

Pricing & Availability
Clone
MOB-47 (See other available formats)
Regulatory Status
RUO
Other Names
IFN-γ Receptor β chain, IFN-γ AF-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
MOB-47
A20 B cell line stained with MOB-47 PE
  • MOB-47
    A20 B cell line stained with MOB-47 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
113603 50 µg 98€
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113604 200 µg 283€
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Description

IFN-γ receptor β chain is also known as IFN-γ AF-1. This 60-65 kD class II cytokine receptor pairs with CDw119 to form the IFN-γ receptor. CDw119 serves as the IFN-γ-binding chain and associates with the IFN-γ β chain which is required for receptor signaling. The extracellular portion of both the IFN-γ receptor α and β chains must be species matched. The IFN-γ receptor β chain is expressed at low levels on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN-γ induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. IFN-γ initiates and regulates a variety of immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse IFN-γR β chain protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunoprecipitation1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113604) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bach EA, et al. 1995. Science 270:1215.
Product Citations
  1. Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
  2. Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed
  3. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  4. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  5. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  6. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  7. Shou P, et al. 2016. Oncogene. 10.1038/onc.2016.128. PubMed
RRID
AB_313560 (BioLegend Cat. No. 113603)
AB_313560 (BioLegend Cat. No. 113604)

Antigen Details

Structure
Class II cytokine receptor family, associates with CDw119, 60-65 kD, AF-1
Distribution

T and B cells, NK cells, monocytes, macrophages, granulocytes, fibroblasts

Function
Regulates immune response, upregulates MHC class I expression
Ligand/Receptor
IFN-γ
Cell Type
B cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Bach EA, et al. 1995. Science 270:1215. 
2. Ansel KM, et al. 2003. Nat. Immunol. 4:616.

Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma R beta chain on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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