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PE anti-mouse JAML Antibody

Pricing & Availability
Clone
4E10 (See other available formats)
Regulatory Status
RUO
Other Names
AMICA
Isotype
Armenian Hamster IgG
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Product Citations
publications
4E10_PE_011909.jpg
BALB/c mouse bone marrow cells (gated on myeloid cell population) stained with 4E10 PE
  • 4E10_PE_011909.jpg
    BALB/c mouse bone marrow cells (gated on myeloid cell population) stained with 4E10 PE
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128503 50 µg 137€
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Description

JAML is a type I integral transmembrane protein with 2 extracellular IgSF domains characteristic of many proteins implicated in cell to cell interactions.JAML is primarily express on granulocytes and reported to be involved in neutrophil migration across tight junctions by its adhesive interaction with epithelial Coxsackie and adenovirus receptor (CAR).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zen K, et al. 2005. Mol Biol Cell 16:2694
Product Citations
  1. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
RRID
AB_2273963 (BioLegend Cat. No. 128503)

Antigen Details

Structure
Type I integral transmembrane protein with 2 extracellular IgSF domains characteristic of proteins implicated in cell-cell interactions, a transmembrane segament and an intracellular tail.
Distribution

Primarily on granulocytes

Ligand/Receptor
Epithelial Coxsackie and adenovirus receptor (CAR)
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Moog-Lutz C et al. 2003. Blood 102 :3371

Gene ID
270152 View all products for this Gene ID
UniProt
View information about JAML on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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